Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

CROP: a feature-independent context-aware method for CRISPR-Cas9 frameshift prediction.

Bioinformatics (Oxford, England)·2026
Same author

NPBIP: predicting binding preferences of uncharacterized nucleic-acid-binding proteins.

Bioinformatics (Oxford, England)·2026
Same author

"Wonderful, wonderful": Functions of praise towards people living with dementia in the acute hospital environment.

Social science & medicine (1982)·2026
Same author

Machine-learning prediction of affinity and epistasis in the bovine pancreatic trypsin inhibitor-chymotrypsin complex.

Protein science : a publication of the Protein Society·2026
Same author

Synthetic RNA-protein decoy granules prevent SARS-CoV-2 infection.

Trends in biotechnology·2026
Same author

Is There Any Dose of Benzodiazepines Safe for Delirium in the PICU? A Secondary Analysis of the PICU Up! Pilot Trial Dataset.

Critical care explorations·2026

相关实验视频

Updated: May 12, 2026

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

64.0K

一个促进真核细胞细胞系基因表达的通用系统.

Inbal Vaknin1, Or Willinger1, Jonathan Mandl2

  • 1Department of Biotechnology and Food Engineering, Technion, Haifa, Israel.

Nature communications
|March 17, 2024
PubMed
概括

我们开发了一种设计算法,使用合成调节元件来促进酵母和哺乳动物细胞的基因表达. 该系统提供了一种优化生物技术中蛋白质生产的新方法.

更多相关视频

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
11:42

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

30.2K
Controllable Ion Channel Expression through Inducible Transient Transfection
10:00

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

9.4K

相关实验视频

Last Updated: May 12, 2026

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

64.0K
High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
11:42

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

30.2K
Controllable Ion Channel Expression through Inducible Transient Transfection
10:00

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

9.4K

科学领域:

  • 合成生物学 合成生物学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 优化基因表达对生物技术至关重要.
  • 现有的转录调节方法可能是复杂的,并且特定于细胞类型.

研究的目的:

  • 开发一种转录调节设计算法,以促进基因表达.
  • 为可预测的基因调节创建一个简化的转录架构.

主要方法:

  • 设计了一种合成上游调节区域 (sURS),使用41种保存的真核生物图案中的最多三种图案.
  • 通过使用大型的原始图书馆 (189,990个变体) 描述了sURS系统.
  • 在酵母和哺乳动物细胞 (CHO-K1,HeLa) 中验证了43个新的sURS设计的表达模型.

主要成果:

  • 奥利戈图书馆的表征产生了一个预测表达式模型.
  • 验证实验证实了模型对未见的sURS设计的准确性.
  • 确定了潜在的通用语法规则,用于酵母中的基因表达增强和减弱,在哺乳动物细胞中也具有功能.

结论:

  • 成功开发和验证了一种用于转录调节的新型设计算法.
  • 合成上游调控区域 (sURS) 系统显示了可预测的基因表达在不同细胞类型中得到增强.
  • 这种算法促进了酵母和哺乳动物系统中工业相关蛋白质的增强表达.