帕斯图雷拉多cida激活Rassf1-Hippo-Yap通路,诱导肺上皮细胞亡
Guangfu Zhao1, Yunhan Tang1, Xiongli Liu1
1College of Veterinary Medicine, Southwest University, Chongqing, China.
Veterinary research
|March 17, 2024
概括
帕斯泰雷拉多种菌感染激活Hippo-Yap通路,导致肺损伤. 用XMU-MP-1抑制这种途径减少了损伤和死亡率,这表明它是巴氏菌病的潜在药物标.
科学领域:
- 病原体与宿主相互作用
- 分子生物学分子生物学
- 呼吸道疾病 呼吸道疾病
背景情况:
- 帕斯图雷拉多达是一种致动物性病原体,导致致命的呼吸道疾病.
- 由P.multocida引起的肺上皮质屏障破坏的机制尚不清楚.
- 在P. multocida感染中Hippo-Yap通路的作用是未知的.
研究的目的:
- 研究Hippo-Yap通路在P. multocida感染中的作用.
- 阐明P.multocida引起的肺损伤的机制.
- 确定巴氏菌病的潜在治疗点.
主要方法:
- RNA-seq分析以确定失调的途径.
- 在体外细胞培养和体内小鼠/子模型.
- 使用XMU-MP-1的Hippo途径的药理抑制.
- 敲击Rassf1以评估其作用.
主要成果:
- P. multocida感染失调并激活了Hippo-Yap路径.
- 激活涉及p-Mst1/2,p-Lats1,p-Yap的上调和下游影响者的下调.
- 拉斯弗1的上调增强了Hippo-Yap通路的活动.
- 治疗XMU-MP-1可以减少细胞亡,肺损伤和死亡率.
- Rassf1的敲击增加了Yap活动,并减少了细胞亡.
结论:
- 菌的感染激活了Rassf1-Hippo-Yap通路,导致肺损伤.
- Rassf1-Hippo-Yap通路是巴氏菌病的潜在治疗标.
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