蛋白激酶C活性调节了HeLa细胞中的核胺A/C动态
Chase C Wesley1, Dallin V North1, Daniel L Levy2
1Department of Molecular Biology, University of Wyoming, 1000 E. University Avenue, Laramie, WY, 82071, USA.
Scientific reports
|March 17, 2024
概括
蛋白质激酶C (PKC) 异形β,但不是α,在相间过程中调节核层中Lamin A/C的运动. 这一发现揭示了PKC在维持细胞分裂之外的核结构方面发挥的新角色.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 核膜提供结构支持,并在核内组织染色质.
- 核层是关键的结构性蛋白质,它们的功能通过翻译后的修饰来调节,例如由蛋白激酶C (PKC) 进行酸化.
- 虽然PKC在线粒分裂中的作用已知,但其在介相期间调节核结构的功能仍然不清楚.
研究的目的:
- 调查蛋白激酶C (PKC) 在调节相间期间核层的动态中的异形特异性作用.
- 为了确定PKC异型α和β是否不同地影响核薄膜内的Lamin A/C流动性.
主要方法:
- 在HeLa细胞中过度表达和击败特定的PKC异型 (PKCα和PKCβ).
- 对Lamin A/C和Lamin B1蛋白水平和局部性的分析.
- 使用光漂白 (FRAP) 或类似技术进行光回收后核外内的Lamin A/C移动部分的量化.
主要成果:
- 过度表达PKCβ,但不增加PKCα,显著增加了核外中的Lamin A/C移动分数.
- Knockdown 的 PKC ß,但不是 PKC α,导致了 Lamin A/C 移动分数的减少.
- 无论是PKC α还是PKC ß的操纵都没有改变核层中Lamin A/C和Lamin B1的整体结构.
结论:
- 蛋白激酶Cβ在细胞周期的相间阶段调节拉胺A/C的动态方面发挥着特定的,依赖于异型的作用.
- 这些发现突出了一个新的机制,PKC通过这种新的机制影响了线粒分裂之外的核结构和色素组织.
相关概念视频
Inhibition of Cdk Activity
4.7K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.7K
MAPK Signaling Cascades
5.5K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.5K
Anaphase Promoting Complex
2.9K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K
Positive Regulator Molecules
5.5K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.5K
PI3K/mTOR/AKT Signaling Pathway
3.5K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.5K
Disassembly of Intermediate Filaments
2.1K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.1K


