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循环RNA HLCS通过miR-338-3p/BPNT1轴调节透镜上皮细胞的亡

Lianyi Sun¹, Fengzhi Li¹, Shuwei Bai²

¹Department of Ophthalmology, Xi'an People's Hospital (Xi'an Fourth Hospital), Shaanxi Eye Hospital, Affiliated Guangren Hospital, School of Medicine, Xi'an Jiaotong University, No. 21 Jiefang Road, Xi'an, 710004, China.

International ophthalmology

|March 17, 2024

概括

循环RNAcirc_HLCS通过抑制亡来防止与年龄相关的白内障 (ARC). 这项研究表明,circ_HLCS通过miR-338-3p/BPNT1通路起作用,这表明circ_HLCS是ARC的潜在治疗标.

科学领域:

眼科医生眼科眼科
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

与年龄相关的白内障 (ARC) 是导致视力受损的主要原因.
目前尚不完全了解ARC病原发生的分子机制.
循环RNAs (circRNAs) 已经成为各种生物过程中的关键调节者.

研究的目的:

研究circ_HLCS在与年龄相关的白内障 (ARC) 的发展中的作用.
阐明circ_HLCS影响白内障形成的分子机制.
探索circ_HLCS作为ARC的治疗点的潜力.

主要方法:

使用qRT-PCR和西方涂抹方法量化circ_HLCS,microRNA (miR)-338-3p和双酸3'-核酶1 (BPNT1) 的量化.
评估紫外线治疗细胞中的细胞增殖,活力和细胞亡.
使用双露西法酶记者和RNA拉下测试来验证向相互作用.

主要成果:

在ARC组织和UV处理的细胞中,circ_HLCS表达显著下降.
过度表达的circ_HLCS受保护于紫外线诱导的细胞增殖和细胞亡的抑制.
circ_HLCS直接针对miR-338-3p,通过这种相互作用调节BPNT1的表达.
通过降低miR-338-3p的调节,通过增加BPNT1表达来逆转紫外线诱导的细胞损伤.

结论:

通过miR-338-3p/BPNT1轴,circ_HLCS通过紫外线处理的细胞抑制细胞灭亡.
circ_HLCS显示出作为与年龄相关的白内障的治疗的潜力.

关键词:

与年龄相关的白内障 BPNT1 BPNT1 的意思是电路_HLCSCS 的情况在 miR-338-3p 中使用.

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