相关实验视频

Updated: Jun 30, 2025

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

由红树林沉积物中丰富的微生物群落对HBCD进行生物转化

Fei Yu¹, Bing Zhang¹, Yongjin Liu¹

¹Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong Province, China.

Journal of hazardous materials

|March 17, 2024

概括

来自红树林沉积物的微生物群落有效地转化1,2,5,6,9,10-Hexabromocyclododecanes (HBCDs),一种持久性有机污染物. 阿尔卡尼沃拉克斯 (Alcanivorax) 细菌利用甲脱酶基因驱动这种生物转化,为环境修复提供了潜在的潜力.

科学领域:

环境科学环境科学
微生物学微生物学
生物化学生物化学

背景情况:

1,2,5,6,9,10 - 六氧化 (HBCDs) 是持久性有机污染物 (POPs),具有环境问题.
红树林沉积物有多样化的微生物群落,具有污染物降解的潜力.

研究的目的:

研究能够转化HBCDs的微生物群落.
确定关键的微生物参与者和参与HBCD生物转化中的酶机制.
探索HBCD污染的生物修复的潜力.

主要方法:

通过连续转移从红树林沉积物中丰富微生物群落.
使用16S rRNA基因测序和基因组结合的细菌组成分析.
对醇甲脱酶基因 (dadAH和dadBH) 的基因表达分析.
使用纯化的 DadAH 和 DadBH 蛋白质进行酶分析.
工程Escherichia coli菌株的构建和测试.

主要成果:

在12个微生物群落中,有6个群体在12代后表现出高的HBCD转化率 (27.5-97.7%).
含有 dadAH 和 dadBH 基因的 Alcanivorax 细菌主导了高转化性社区.
观察到HBCD诱导的dadAH和dadBH的表达.
纯化的 DadAH 和 DadBH 酶显示出高的 HBCD 转化率 (分别为 91.9% 和 101.0%).
与无细胞提取物相比,工程化大肠杆菌菌株的HBCD转化效率较低.
转化途径包括脱,水解和还原性脱.

结论:

微生物群落,特别是Alcanivorax,可以有效地转化HBCDs.
甲脱酶 DadAH 和 DadBH 是 HBCD 生物转化中的关键酶.
该研究为针对HBCD污染的生物修复策略提供了基础.

关键词:

生物转化生物转化脱原酶是一种脱原酶.在 HBCD 中,微生物社区的微生物社区.

更多相关视频

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Published on: October 31, 2019

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Published on: February 10, 2023

相关概念视频

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

What are Biogeochemical Cycles?

What are Biogeochemical Cycles?

The most common elements in organic molecules, carbon, hydrogen, oxygen, nitrogen, sulfur, and phosphorus, are only available in the ecosystem in limited amounts. Therefore, these nutrients must be recycled through both biotic and abiotic components of the ecosystem, in processes generally called biogeochemical cycles.