通过阻断Wnt信号的过度激活,Matrine在感染eimeria necatrix的小中减少了肠道干细胞损伤
Geng-Xiu Zan1, Xiao-Fan Wang1, Shao-Kang Yan1
1College of Animal Science, South China Agricultural University/State Key Laboratory of Swine and Poultry Breeding Industry/Guangdong Laboratory for Lingnan Modern Agriculture/Guangdong Provincial Key Laboratory of Animal Nutrition Control/State Key Laboratory of Swine and Poultry Breeding Industry, Guangzhou 510642, China.
概括
马特林及其化合物AntiC通过调节Wnt/β-catenin信号传递来保护肠免受Eimeria necatrix (EN) 感染. 这些化合物促进肠道干细胞的分化,提供一种营养策略来防止EN诱导的损伤.
科学领域:
- 禽畜科学 禽畜科学 禽畜科学
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
背景情况:
- 由Eimeria necatrix (EN) 引起的菌病是一种重要的家禽肠道疾病.
- 马特林是一种天然化合物,具有抗虫和抗炎性质.
研究的目的:
- 评估Matrine和AntiC在小肠中对EN感染的保护作用.
- 探索对因EN引起的肠损伤的营养干预策略.
主要方法:
- 在体内研究:392只肉被分配到对照组,Matrine,AntiC,EN,EN+Matrine和EN+AntiC组.
- 试验室研究:小肠道器官 (IOs) 用Matrine,AntiC或EN.进行了治疗.
- 通过组织学,TEM,qPCR,Westernblotting和免疫组织化学,评估了阴茎隐形轴完整性,肠干细胞 (ISC) 活性和Wnt/β-catenin信号传递.
主要成果:
- 在感染EN的小中,补充Matrine和AntiC改善了小体形态 (小体高度,密室增大) 和紧接蛋白表达.
- 马林和AnticC抑制了EN诱导的Wnt/β-catenin信号激活.
- Wnt/β-catenin通路的激活阻断了Matrine和AntiC对受损ISC的保护作用.
结论:
- 马特林和AnticC通过抑制ISC增殖和通过Wnt/β-catenin通路调节促进分化来减轻EN损伤.
- 这些发现为使用Matrine和AntiC作为用于禽类因EN引起的肠损伤的营养干预措施提供了新的见解.
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