脱氧胆酸通过抑制MAPK信号通路来抑制ASFV复制
Qi Gao1, Yifan Xu2, Yongzhi Feng2
1Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; African Swine Fever Regional Laboratory of China (Guangzhou), Guangzhou 510642, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou 510000, China.
International journal of biological macromolecules
|March 17, 2024
概括
非洲猪瘟病毒 (ASFV) 感染激活MAPK通路,但DCA通过阻止这种激活来抑制病毒复制. 这项研究为开发针对ASFV的新抗病毒药物提供了基础.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 非洲猪瘟 (ASF) 是一种高度传染性的猪病毒性疾病.
- 了解ASFV的分子机制对于抗病毒药物开发至关重要.
研究的目的:
- 为了阐明ASFV感染的分子机制.
- 评估二乙酸 (DCA) 在ASFV感染期间对MAPK通路的影响.
- 为新的抗病毒疗法提供科学基础.
主要方法:
- 使用了转录组和代谢组分析.
- 双露西法酶记者测定确定了影响MAPK通路的ASFV基因.
- 同焦显微镜可视化了蛋白质相互作用和细胞定位.
主要成果:
- ASFV感染对IL-17和MAPK信号通路进行上调,促进病毒复制.
- 感染后DCA水平增加;外源DCA抑制ASFV诱导的MAPK激活和复制.
- ASFV蛋白质I73R上调调节AP-1转录,促进其核进入,并与AP-1相互作用,所有这些都是DCA抑制的.
结论:
- DCA通过抑制MAPK通路的ASFV介导激活,有效地抑制ASFV复制.
- 这些发现为研究ASF病变和开发新型抗病毒治疗提供了理论基础.
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