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一个从Tetrastigma hemsleyanum中分离出来的葡萄糖黄糖丹Diels和Gilg:结构和免疫调节活性

Zian Mao¹, Liu Yang¹, Yishan Lv¹

¹School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 548 Binwen Rd., Hangzhou, Zhejiang 310053, China.

Carbohydrate polymers

|March 17, 2024

概括

来自Tetrastigma hemsleyanum的一种新型酸性多糖体STHP-5刺激巨细胞,并作为Toll-like受体4 (TLR4) 激动剂,表明其作为免疫调节剂的潜力.

科学领域:

自然产品化学自然产品化学
免疫学免疫学免疫学
葡萄糖科学 (Glycoscience) 是一种科学.

背景情况:

德尔斯·吉尔格 (Diels et Gilg) 是一种生物活性化合物的植物来源.
多糖因其多样化的生物活动而闻名,包括免疫调节.
了解植物性多糖的结构-活性关系对于开发新疗法至关重要.

研究的目的:

从Tetrastigma hemsleyanum中分离和描述一种新型的酸性多糖化物 (STHP-5).
在体外研究STHP-5的免疫调节作用.
为了确定STHP-5是否作为托尔类受体4 (TLR4) 激动剂.

主要方法:

从Tetrastigma hemsleyanum中分离和净化STHP-5的方法.
分子重量测定和链形状分析.
组合分析 (银糖,曼诺糖,葡萄糖酸) 和糖酸链接分析 (甲基化,NMR).
在体外测试中使用RAW264.7细胞测量氧化 (NO) 和细胞因子 (IL-6,MCP-1,TNF-α) 的产生.
用HEK-BlueTM-hTLR4细胞测定来评估TLR4的激素活性和EC50的确定.

主要成果:

隔离了一种新型的酸性葡萄糖甲曼南 (STHP-5) ,其分子量为3.225 × 10^5 kDa.
STHP-5呈现出随机的线圈链形状,主要由银河糖,曼和葡萄糖酸组成.
在实验室中,STHP-5刺激了巨细胞,诱导氧化和促炎性细胞因子分泌 (IL-6,MCP-1,TNF-α).
STHP-5表现出TLR4激动剂活性,纯化的多糖体显示出比原始多糖体更强的激活 (较低的EC50).

结论:

STHP-5是一种从Tetrastigma hemsleyanum中分离出来的新型酸性多糖体.
STHP-5具有显著的免疫调节特性,作为巨细胞刺激剂和TLR4激动剂.
这些发现表明,STHP-5具有作为治疗应用的新型免疫调节剂的潜力.

关键词:

免疫调节活性免疫调节活性它是一种多糖化合物.结构特征结构特征结构特征刺斑 (Tetrastigma hemsleyanum) 是由迪尔斯和吉尔格 (Diels et Gilg) 制造的一种.

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