相关实验视频

Updated: Jun 30, 2025

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Published on: January 26, 2017

在Phytophthora infestans ATP6基因的两个同名突变之间的适应性差异

Oswald Nkurikiyimfura¹, Abdul Waheed¹, Hanmei Fang¹

¹Institute of Plant Virology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.

BMC ecology and evolution

|March 18, 2024

概括

ATP6基因中的同名突变影响了Phytophthora infestans的健康和适应. 这些遗传变异对温度敏感性等特征产生影响,影响病原体的分布和管理策略.

科学领域:

进化生物学进化生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

以前被认为是中性的同义突变,越来越多地被认为是它们在物种适应中的作用.
有证据表明同义突变调节遗传信息流动并影响功能特征.
这项研究调查了Phytophthora infestans.内部的家政基因ATP6中同名突变的作用.

研究的目的:

分析Phytophthora infestans中ATP6基因的序列特征.
为了在同名突变中比较健康成分,包括新陈代谢率,温度敏感性,攻击性和真菌杀菌剂耐受性.
了解关键植物病原体中同名突变的适应潜力.

主要方法:

从139个Phytophthora infestans分离物中分析ATP6基因序列.
在已识别的同名突变体之间比较与健康相关的特征.
评估温度介导的空间分布和基因表达模式.

主要成果:

在ATP6基因中观察到较低的遗传变异,两种主要的同名突变物存在于相似的频率.
单个核酸替代使这些突变异化,导致适应性和温度依赖性特征的显著差异.
一种突变 (AT结尾) 在较冷的地区普遍存在,在较低的温度下表达更高,而GC结尾突变则表现出相反的模式.

结论:

同名突变可以显著调节物种的适应性进化,包括病原体.
这些发现对可持续的疾病管理有影响,特别是在气候变化的背景下.
同名突变有助于生物的功能适应,而不仅仅是改变蛋白质序列.

关键词:

疾病管理疾病管理一种物种的适应性.中立理论中立理论这种类型的 Oomycete.植物病原体的病原体人口遗传学人口遗传学热介导的适应方式

更多相关视频

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases

Published on: November 22, 2024

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

相关概念视频

ATP Synthase: Structure

ATP Synthase: Structure

ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...

ATP Synthase: Mechanism

ATP Synthase: Mechanism

In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...

ATP Driven Pumps I: An Overview

ATP Driven Pumps I: An Overview

ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...

Mutations

Mutations

Complementation Tests

Complementation Tests

A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...