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Updated: Jun 30, 2025

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

全基因组识别参与亚托皮性皮肤炎的失调替代拼接和RNA结合蛋白

Yaqi Yang¹, Hao Chen¹, Qing Jiang¹

¹Department of Allergy, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.

Frontiers in genetics

|March 18, 2024

概括

调节失调的RNA结合蛋白 (RBPs) 和它们的替代拼接事件 (RASEs) 涉及到亚托皮炎 (AD) 病因. 升级的RBP和改变的RASE,特别是在免疫路径中,表明AD的潜在治疗点.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
免疫皮肤学免疫皮肤学

背景情况:

亚托匹性皮肤炎 (AD) 的发病过程涉及复杂的分子机制.
RNA结合蛋白 (RBPs) 和替代拼接事件 (RASEs) 越来越多地被认为是它们在疾病中的作用.
了解阿尔茨海默病中RBPs和RASEs的相互作用对于确定治疗点至关重要.

研究的目的:

调查RBPs和RASEs在阿托皮性皮肤炎分子机制中的作用.
为了确定在AD皮肤病变和外周血液中失调的特定RBP和RASE.
探索这些分子作为AD的治疗点的潜力.

主要方法:

来自健康,非损伤和损伤AD皮肤样本的RNA测序数据的分析.
鉴定差异表达基因 (DEG),RBP (DE-RBP) 和替代拼接事件 (ASE).
通过qPCR构建共表达网络,并通过qPCR验证外围血液单核细胞 (PBMC) 中的基因表达.

主要成果:

确定了60个DE-RBPs,主要在病变AD皮肤上升调节,并与免疫和亡途径相关.
观察到不同的ASEs和RASEs,在AD组中alt3p和alt5p是显著的.
在AD患者的PBMC中验证了四个基因 (IFI16,S100A9,PKM,ENO1) 的升级调节,并在DDX中增加了RASEs60.

结论:

失调的RBP及其相关的RASE在亚托皮性皮肤炎的发展中发挥着重要作用.
参与免疫和细胞骨通路的特定RBP和RASE是AD病变发生的关键因素.
这些已识别的分子参与者代表了阿托皮性皮肤炎的有希望的潜在治疗点.

关键词:

RNA结合蛋白质是RNA结合的蛋白质.替代性拼接是一种替代性的拼接.亚托邦性皮肤炎的发生.进行全基因组分析.免疫和炎症反应的反应.

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