Search research articles

相关概念视频

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Machine-Learning-Based Automated Schlemm's Canal Volumetric Segmentation for Optical Coherence Tomography.

Chemical & biomedical imaging·2026

Same author

Vis-OCT Explorer: an open-source software for visible-light optical coherence tomography data processing.

Biomedical optics express·2026

Same author

The dynamics of nucleolus-centromeres interaction in living cells.

bioRxiv : the preprint server for biology·2026

Same author

Super-resolution functional photoacoustic microscopy via label-free cell tracking.

Light, science & applications·2026

Same author

PROX1 loss in adult mouse Schlemm's canal causes permanent ocular hypertension.

bioRxiv : the preprint server for biology·2025

Same author

Optical strategies for in vivo retinal ganglion cell imaging.

Med-X·2025

Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026

Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026

Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026

Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026

Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026

Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026

查看所有相关文章

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

Search research articles

相关实验视频

Updated: May 10, 2026

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Published on: September 29, 2017

使用三维单分子局部化显微镜量化与线粒体功能障碍相关的纳米变化.

Benjamin Brenner¹, Fengyuanshan Xu¹, Yang Zhang^1,2

¹Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.

Biomedical optics express

|March 18, 2024

概括

此摘要是机器生成的。

我们开发了一种新的方法,通过3D超高分辨率显微镜图像量化线粒体形状和蛋白质密度. 这种工具可以对单个线粒体进行详细的分析,这对于了解细胞健康和疾病至关重要.

更多相关视频

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Published on: January 17, 2025

相关实验视频

Last Updated: May 10, 2026

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Published on: September 29, 2017

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Published on: January 17, 2025

科学领域:

细胞生物学细胞生物学
显微镜的使用方法
生物物理学的生物物理.

背景情况:

线粒体形态反映了细胞的完整性和功能.
3D超分辨率显微镜允许对线粒体特征进行个别分析.
从这些图像中提取形态参数的现有工具是有限的.

研究的目的:

开发一种从3D超分辨率显微镜图像中提取线粒体形态学指标的定量方法.
为了使单个线粒体在形态分析中的敏感性.
为分析疾病背景中的线粒体变化提供一种工具.

主要方法:

对3D单分子局部化显微镜 (SMLM) 图像进行定量分析.
提取包括体积,面积比和局部蛋白质密度在内的指标.
使用模拟的SMLM数据和经过实验改变的线粒体进行验证.

主要成果:

建立了一种用于定量线粒体形态分析的新方法.
该方法证明了单个线粒体的敏感性.
验证证实了提取的形态参数的准确性.

结论:

这项工作为线粒体形态学的详细,定量分析提供了至关重要的工具.
开发的方法有助于研究单个线粒体的变化.
它为分析单细胞水平上与疾病相关的线粒体变化铺平了道路.