Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.0K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Learning millisecond protein dynamics from what is missing in NMR spectra.

bioRxiv : the preprint server for biology·2026
Same author

Untrained Position-Encoded Multilayer Perceptron Network for Structured Illumination Microscopy Reconstruction.

Chemical & biomedical imaging·2026
Same author

Isotropic shrinkage of patterned vacancies enables three-dimensional nanoprecise metastructures for visible light applications.

Nature photonics·2026
Same author

QuATON: quantization aware training of optical neurons.

Optics express·2026
Same author

Comprehensive 16S rRNA gene sequencing and meta-transcriptomic analyses of the female reproductive tract microbiota: two molecular profiles with different messages.

Human reproduction open·2026
Same author

Acute high-dose irradiation disrupts cell adhesion and Silk-Ovarioid formation in human primary ovarian cells.

Journal of ovarian research·2026

相关实验视频

Updated: Jun 30, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.6K

通过微分显微镜进行超快速定量相位成像 [受邀]

Udith Haputhanthri1,2, Kithmini Herath1,2, Ramith Hettiarachchi1,2

  • 1Center for Advanced Imaging, Faculty of Arts and Sciences, Harvard University, Cambridge, MA 02138, USA.

Biomedical optics express
|March 18, 2024
PubMed
概括

量相显微镜 (QPM) 现在可以使用一种新的光学压缩-解压缩框架来实现更高的吞吐量. 这种方法减少了数据采集瓶,为各种应用程序提供更快的无标签成像.

更多相关视频

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

11.4K
Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
07:38

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

Published on: September 13, 2016

8.4K

相关实验视频

Last Updated: Jun 30, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.6K
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

11.4K
Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
07:38

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

Published on: September 13, 2016

8.4K

科学领域:

  • 生物医学光学 生物医学光学
  • 显微镜技术 显微镜技术
  • 计算成像技术的成像

背景情况:

  • 定量相显微镜 (QPM) 是一种无标签的成像技术,在代谢学和组织病理学中具有广泛的应用.
  • 目前的QPM吞吐量受到图像传感器像素率的限制,这阻碍了实时分析和大体积研究.
  • 传感器和深度学习的进步改善了QPM,但没有克服基本的硬件瓶.

研究的目的:

  • 在QPM中开发一个新的压缩图像采集框架,以克服硬件限制.
  • 引入可学习的光学压缩解压缩系统,以提高QPM吞吐量.
  • 通过内容特定的功能学习来证明QPM的显著吞吐量改进.

主要方法:

  • 微分定量相显微镜 (∂-QPM) 系统的数值模拟.
  • 在硬件层面实现可学习的光学处理器用于图像压缩.
  • 使用重建神经网络进行后获取图像解压缩.

主要成果:

  • 在数值实验中实现了 ×64 的压缩系数.
  • 保持了高图像保真度,结构相似度指数 (SSIM) 为0.90.
  • 保持图像质量,用于蜂成像的峰值信号噪声比 (PSNR) 为30dB.

结论:

  • 拟议的 ∂-QPM 框架为显著提高 QPM 系统吞吐量提供了一条新的途径.
  • 学习光学压缩有效地绕过传感器像素率的限制.
  • 这种方法有望为无标签,高通量显微镜带来前所未有的改进.