基于罗达胺的光探针用于动态的线粒体STED成像的线粒体
Xinwei Gao1, Songtao Cai1, Luwei Wang1
1Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Biomedical optics express
|March 18, 2024
概括
研究人员开发了一种新的光探针 (TPERh) 用于刺激辐射消耗 (STED) 显微镜,克服了防漂白的限制. 这种探测器可以对线粒体结构进行详细的成像,有助于疾病研究.
科学领域:
- 生物医学成像学 生物医学成像学
- 超高分辨率显微镜的使用方法
- 光探测器发展发展
背景情况:
- 刺激发射耗尽 (STED) 显微镜具有显著的生物医学潜力.
- 当前STED探针的弱反漂白性能限制了它们的应用.
- 具有激发波长在500-600 nm之间的STED探针的有限可用性.
研究的目的:
- 开发一种具有增强抗漂白性能的新型STED光探针.
- 针对线粒体动态晶体进行超分辨率成像.
- 为了弥补STED探测器在500-600nm波长范围内运行的差距.
主要方法:
- 一种新的四乙烯乙烯功能化罗达胺染料 (TPERh) 的合成.
- 评估TPERh探针的抗漂白性能和和刺激辐射功率.
- 对线粒体STED超分辨率成像和线粒体变形分析的TPERh的应用.
主要成果:
- TPERh 探测器显示出优异的抗漂白性能.
- 对TPERh探测器观察到低和刺激辐射功率.
- 探测器成功地以高分辨率成像了线粒体动态晶体.
结论:
- TPERh 探针是生物医学中先进的线粒体成像的一个有前途的工具.
- 它的优越抗漂白能力扩大了STED显微镜的应用.
- TPERh促进了与疾病相关的线粒体形态学的研究.
更多相关视频
06:57Author Spotlight: Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells
Published on: May 12, 2023
7.7K
07:53Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
Published on: June 4, 2020
7.2K
相关概念视频
01:19Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K