Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

SAMJ: fast image annotation on ImageJ/Fiji via segment anything model.

Nature communications·2026
Same author

Search, organize, aggregate and share image data with BioFile Finder (BFF).

Nature methods·2026
Same author

Adherence to risk minimization measures for alemtuzumab use in multiple sclerosis: a drug utilization study in four European countries.

Therapeutic advances in neurological disorders·2026
Same author

Gelatin Methacryloyl Hydrogels as a Multifaceted Platform for Promoting Nerve Regeneration.

Tissue engineering. Part B, Reviews·2026
Same author

Ecological and health risk assessment of rare earth elements in Kaifeng, Henan Province: a Monte Carlo probabilistic approach.

Scientific reports·2026
Same author

Modification of CoFe Prussian Blue Structure by N<sub>2</sub> Plasma for Enhanced Electrocatalysis.

Materials (Basel, Switzerland)·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
查看所有相关文章

相关实验视频

Updated: Jun 30, 2025

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

46.2K

使用动态光学连贯显微镜评估细胞活力.

Chao J Liu1, Jason T Smith1, Yuanbo Wang1

  • 1Elephas Biosciences Corporation, 1 Erdman Place, Madison, WI 53717, USA.

Biomedical optics express
|March 18, 2024
PubMed
概括
此摘要是机器生成的。

动态光学连贯显微镜 (dOCM) 提供了一种无标签的方法来评估细胞活力. 这种技术可以准确地区分瘤中的活细胞和死细胞,并与代谢成像相关,促进癌症研究和治疗评估.

更多相关视频

Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment
10:13

Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment

Published on: June 21, 2022

2.2K
Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
12:12

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

Published on: December 5, 2020

6.1K

相关实验视频

Last Updated: Jun 30, 2025

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

46.2K
Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment
10:13

Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment

Published on: June 21, 2022

2.2K
Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
12:12

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

Published on: December 5, 2020

6.1K

科学领域:

  • 生物医学光学 生物医学光学
  • 细胞代谢成像 细胞代谢成像
  • 癌症研究 癌症研究

背景情况:

  • 准确的细胞活力评估在各个研究学科中至关重要.
  • 使用光染料的现有光学方法在可靠性和组织透方面存在局限性.
  • 动态光学连贯显微镜 (dOCM) 提供基于细胞代谢变化的无标签对比.

研究的目的:

  • 为了比较无标签的dOCM与传统的可活性染料的对比.
  • 为了证明dOCM在复杂的生物样本中区分活细胞和死细胞的能力.
  • 为了确定dOCM实时对比和细胞代谢状态之间的相关性.

主要方法:

  • 使用动态光学连贯显微镜 (dOCM) 进行无标签成像.
  • 在生物样本中比较dOCM与标准可活性染料的对比.
  • 在初级小鼠肝脏组织中使用光学氧化还原比进行了代谢成像.

主要成果:

  • dOCM成功地将活细胞与死细胞区分在小鼠神经基因瘤中,这是一个新的发现.
  • 在dOCM实时对比和肝脏组织中的光学氧化还原比之间观察到强烈的相关性.
  • 无标签的dOCM提供了可靠的对比度,反映了细胞代谢状态.

结论:

  • dOCM是一种有前途的无标签成像工具,用于在研究环境中评估细胞活力.
  • 该技术可用于评估各种癌症治疗的疗效,包括免疫瘤学和化疗.
  • dOCM提供了一条新的途径,用于分析没有外源标签的活瘤组织.