面向一个迷你内镜设计,具有空间选择性激发和成像
Loïc Tabourin1,2,3, Frédéric Bretzner2,4,5, Tigran Galstian1,6
1Center for Optics, Photonics and Lasers (COPL), Faculty of Science and Engineering, Department of Physics, Engineering Physics and Optics, Université Laval, Québec, QC G1V 0A6, Canada.
Biomedical optics express
|March 18, 2024
概括
这项研究引入了一种新的迷你内镜,配备液晶透镜阵列,用于动态分辨率增强. 这一进步使得在没有机械部件的情况下,可以在显微镜中进行改进的成像.
科学领域:
- 生物医学工程 生物医学工程
- 在光学成像系统中,光学成像
- 显微镜技术 显微镜技术
背景情况:
- 传统的迷你内镜通常需要机械元件来聚焦,从而限制了它们的适应性.
- 在大视野 (FoV) 上同时实现高空间分辨率是内镜成像的一个重大挑战.
研究的目的:
- 介绍一款新的迷你内镜设计,采用电调液晶透镜阵列.
- 为了证明空间分辨率和工作距离在没有机械运动的情况下的动态调整.
- 为了评估微观成像应用的迷你内镜的性能.
主要方法:
- 设计和实施一个采用电调液晶透镜阵列的迷你内镜.
- 使用均的光膜,光微球和表达GCaMP6s的组织样本进行了表征.
- 评估横向分辨率和视野性能.
主要成果:
- 通过改变工作距离,证明了空间分辨率的动态调整.
- 实现了高达2微米的侧面分辨率.
- 在300微米至400微米的视野中确认了功能.
结论:
- 开发的迷你内镜可以在没有机械部件的情况下动态控制空间分辨率.
- 这项技术可以为各种微观应用提供增强的成像功能.
- 该系统有望在生物和医学领域改善可视化.
相关概念视频
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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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