植物生长调节剂通过NaCl应激减轻苗根部的氧化损伤
Yaxin Wang1, Li-Ming Zhao1, Naijie Feng1,2,3
1College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong, China.
PeerJ
|March 18, 2024
概括
叶片喷雾的5-aminolevulinic 酸 (5-ALA) 和2-Diethylaminoethyl 六酸 (DTA-6) 改善了水苗的盐耐受性. 与DTA-6相比,5-ALA在减轻NaCl诱导的根损伤方面表现出更好的疗效.
科学领域:
- 农业科学 农业科学
- 植物生理学 植物生理学
- 生物化学 生物化学
背景情况:
- 米 (Oryza sativa L.) 是一个重要的全球粮食作物,易受土壤盐度的影响.
- 盐应激通过破坏生理和生化过程,对植物生长产生负面影响.
- 植物生长调节剂的外源应用可以提高作物应激耐受性.
研究的目的:
- 为了评估5-氨基诺列维林酸 (5-ALA) 和2-二甲基氨基乙醇六酸 (DTA-6) 在改善米盐耐受性的疗效.
- 调查5-ALA和DTA-6对大米根中NaCl应激的保护作用背后的生理和生化机制.
- 为了比较5-ALA和DTA-6在减轻盐引起的损害方面的相对有效性.
主要方法:
- 米苗 ("黄华山") 接受了六种治疗:对照,5-ALA,DTA-6,NaCl,5-ALA + NaCl,以及DTA-6 + NaCl.
- 在三叶一心阶段进行了5-ALA (40 mg/L) 和DTA-6 (30 mg/L) 的叶子应用.
- 在NaCl处理后的不同时间点 (0.3%W/W) 分析了水根的形态和生理-生化指数,包括氧化应激标志物和激素水平.
主要成果:
- NaCl压力显著抑制了大米的生长,增加了氧化应激标志物 (MDA,H2O2,O2) 和改变了激素平衡.
- 在盐应激下进行的5-ALA和DTA-6治疗都增强了抗氧化酶活性,透调节物质含量和非酶性抗氧化剂 (AsA,GSH).
- 5-ALA和DTA-6降低了ROS积累和MDA含量,调节了内源激素水平 (增加GA3,JA,IAA,SA,ZR;减少ABA),而5-ALA显示出比DTA-6更明显的保护作用.
结论:
- NaCl压力对大米根系产生不利影响.
- 对5-ALA和DTA-6的叶子应用有效地减轻了根中NaCl诱导的损伤.
- 在提高米盐耐受性方面,5-ALA比DTA-6更有效.
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