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对具有强烈抗瘤活性的结合粘附分子-a产生和表征mAb 61H9

Kang Liu^1,2, Hang Yang², Rong Xiong²

¹State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu, Sichuan, China.

|March 18, 2024

概括

研究人员开发了一种高亲和度单克隆抗体 (mAb),该抗体针对结合粘附分子-A (JAM-A). 这种JAM-A mAb有效地抑制了食道状细胞癌 (ESCC) 的进展和小鼠的瘤生长.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

结合粘附分子-A (JAM-A) 是一种在各种免疫和内皮细胞上发现的粘附分子.
JAM-A在细胞与细胞相互作用中发挥作用,并对癌症生物学有影响.

研究的目的:

开发一种针对JAM-A.A.的高亲和度单克隆抗体 (mAb).
评估开发的JAM-A mAb对食道状细胞癌 (ESCC) 的抗瘤作用.

主要方法:

对哺乳动物表达的JAM-A-FccccDNA的化学合成和优化.
混合瘤细胞系的生成和选,以产生抗JAM-A mAbs.
包括免疫光,流细胞计和*in vivo*瘤模型在内的测试,以评估mAb的疗效.

主要成果:

获得了四个单克隆菌株,其中61H9显示了最强的信号和对JAM-A.的高度亲和力.
反JAM-A mAb显著减弱了ESCC细胞的增殖,迁移和入侵.
在接受抗JAM-A mAb治疗的小鼠中,瘤生长被显著抑制,BCL-2和IκBα表达减少.

结论:

建立了一种高产量的JAM-A-Fc蛋白质生产方法.
成功制备了一种具有显著抗瘤活性的高亲和度抗JAM-A mAb.
该JAM-A mAb证明了作为ESCC的治疗剂的潜力.

关键词:

这是一个反-JAM-A.食道癌是食道癌的一个类型.混合体瘤是一种混合体瘤.结点粘附分子-A 的结点粘附分子-A.单克隆抗体是一种单克隆抗体.

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