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Updated: Jun 30, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

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修订的氧尿素诱导细胞循环停止的机制和改进的替代方案

Alisa E Shaw1, Jackson E Whitted1, Mattias N Mihelich1

  • 1Department of Biochemistry and Molecular Biology, Colorado State University, CO, USA.

bioRxiv : the preprint server for biology
|March 18, 2024
PubMed
概括

氧尿素 (HU) 通过产生活性氧物种 (ROS) 导致细胞循环停止,这些活性氧物种抑制DNA聚合酶,独立于Mrc1检查点. 一个新的工具,RNR-deg,为研究复制应激提供了一个更安全的替代方案.

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