替代拼接对Arabidopsis蛋白质组的影响
Andres V Reyes1,2, Ruben Shrestha1, TaraBryn S Grismer1,2
1Division of biosphere science and engineering, Carnegie Institution for Science, Stanford, California, USA.
bioRxiv : the preprint server for biology
|March 18, 2024
概括
替代拼接,特别是内部保留,影响植物蛋白质组. 这项研究揭示了内部保留对蛋白质多样性的影响小于预期,但影响蛋白质水平和植物表型.
科学领域:
- 植物分子生物学 植物分子生物学
- 蛋白质组学是指蛋白质组学.
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 替代拼接 (AS) 是一个关键的真核生物调节过程.
- 内部保留是植物中占主导地位的AS类型.
- 阿斯对植物蛋白质组的全球影响仍未得到充分研究.
研究的目的:
- 为了研究改变的替代拼接对阿拉比多普西斯蛋白质组的影响.
- 分析剪接因子ACINUS和PININ的变化如何影响蛋白质的丰富性和多样性.
- 整合蛋白质组和转录组数据以了解内子保留效应.
主要方法:
- 用实时搜索MS3 (RTS-SPS-MS3) 进行高覆盖蛋白质定量化.
- 为了准确的蛋白质组分析,采用了广泛的样本分化方法.
- 整合蛋白质组数据与转录组数据,以将拼接变化与蛋白质水平变化相关联.
主要成果:
- 观察到转录和蛋白质水平变化之间存在微弱至中度的相关性.
- 证明增加的内质保留 (IIR) 可以显著影响蛋白质水平,但对蛋白质多样性的影响小于预期.
- 确定突变物中的IIR事件在压力条件下的野生类型植物中也存在.
- 与观察到的表型相关的集体蛋白质变化,如改变的安东素水平和生长缺陷.
结论:
- 内部保留在调节植物中的蛋白质丰度方面起着复杂的作用.
- 内质保留对蛋白质多样性的影响不如其对蛋白质水平的影响.
- 蛋白质学见解对于理解与拼接因子破坏相关的植物表型至关重要.
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