在斑马鱼胚胎发生过程中,新出现的H3K9me3染色质景观
Katherine L Duval1, Ashley R Artis1, Mary G Goll1
1Department of Genetics, University of Georgia, Athens, GA, USA.
bioRxiv : the preprint server for biology
|March 18, 2024
概括
斑马鱼胚胎通过3氨酸9三甲基化 (H3K9me3) 标记建立异质染色素,主要准发育早期的LTR转位子. 这一过程始于胚胎基因组激活之前,并在胚胎发生过程中迅速扩大.
科学领域:
- 发育生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 细胞的基因组组织依赖于euchromatin和 heterochromatin.
- 在胚胎发生过程中对异染色质的建立的理解是有限的.
- 基因组3氨酸9三甲基化 (H3K9me3) 对于异性染色素的形成至关重要.
研究的目的:
- 在斑马鱼胚胎中定义新兴的H3K9me3景观.
- 了解早期发育过程中H3K9me3沉积的时间动态.
主要方法:
- 使用CUT&RUN (在目标下切割和释放) 来进行H3K9me3.3的高分辨率映射.
- 在不同发育阶段的斑马鱼胚胎中分析了H3K9me3模式.
主要成果:
- 确定了LTR转位子作为胚胎H3K9me3.3.的首选标.
- 发现了早期的H3K9me3位点,富含母体piRNA和周心组序列,先于胚胎基因组激活 (ZGA).
- 在ZGA期间观察到H3K9me3部位的线性增加和在已建立的部位的快速信号增加>10倍.
结论:
- 提供了斑马鱼胚胎发生过程中新出现的H3K9me3的高分辨率时间地图.
- 揭示了LTR转子和与piRNAs和基因组位置相关的早期H3K9me3建立的优先向.
- 显示了快速,动态的H3K9me3积累,与ZGA和正在进行的表观遗传重塑相吻合.
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