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通过用小分子调节单个纳米颗粒蛋白冠状体来进行深度等离子体蛋白质谱分析

Ali Akbar Ashkarran¹, Hassan Gharibi², Seyed Amirhossein Sadeghi³

¹Department of Radiology and Precision Health Program, Michigan State University, East Lansing, MI 48824, USA.

bioRxiv : the preprint server for biology

|March 18, 2024

概括

添加到血中的小分子显著增强纳米粒子蛋白冠状分析. 这改善了血蛋白质组的分析,使得检测到更多的蛋白质和潜在的生物标志物用于诊断.

科学领域:

生物分子分析.
纳米技术纳米技术
蛋白质组学是指蛋白质组学.

背景情况:

纳米粒子 (NP) 上的蛋白质冠状体是使用液态染色体质谱法 (LC-MS/MS) 进行等离子体蛋白质组分析的关键.
目前的方法在检测血蛋白的全谱方面存在局限性.

研究的目的:

调查小分子是否可以改变蛋白质冠状形成并增强血蛋白质覆盖范围.
建立使用NP和小分子进行等离子体蛋白质组分析的新标准.

主要方法:

将聚乙烯NP暴露在与各种小分子 (代谢物,脂质,维生素) 添加的血中.
使用LC-MS/MS和数据独立获取 (DIA) 蛋白质组学分析蛋白质冠状.
使用分子动力学模拟来研究NP-蛋白相互作用.

主要成果:

与单独的血相比,尖的小分子增加了蛋白质检测的8.25倍.
单独的甲可检测897种蛋白质,并通过耗尽丰富的蛋白质来降低动态范围.
通过酸胆优化的DIA检测到1436种蛋白质,而自上而下的蛋白质组学确定了337种额外的蛋白质形式.

结论:

将小分子添加到血中是一种强大的策略,可以增强基于NP的蛋白质组概况.
这种方法显著增加可检测的血蛋白和蛋白质的深度和宽度.
该方法为生物标志物发现和临床翻译提供了一种简化,单NP,单分子方法.

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