光和多巴胺对两个昼夜神经元产生影响,促进早晨的清醒
Jasmine Quynh Le1, Dingbang Ma1,2, Xihuimin Dai1
1Howard Hughes Medical Institute and Department of Biology, Brandeis University, Waltham, Massachusetts 02453, USA.
bioRxiv : the preprint server for biology
|March 18, 2024
概括
光能通过特定大脑神经元中的多巴胺信号来刺激的早晨清醒. 在表达离子运输 (ITP) 的神经元中的多巴胺受体1 (Dop1R1) 是这种依赖光的反应的关键.
科学领域:
- 时间生物学 时间生物学
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 循环节律调节哺乳动物和的睡眠-觉醒周期.
- 特定的大脑神经元,包括表达离子运输 (ITP) 的侧面神经元 (LNs),控制这些节奏.
- 多巴胺信号传递影响了和哺乳动物的活动.
研究的目的:
- 调查多巴胺信号传递在ITP表达LNS中的作用.
- 为了确定多巴胺受体Dop1R1在早晨活动中的功能.
- 为了阐明在中早晨清醒的依赖光的机制.
主要方法:
- 单细胞测序以确定昼夜神经元组.
- 通过CRISPR/Cas9突变发生,在ITP+ LNs中特别改变Dop1R1.
- 活体成像测量cAMP水平作为对多巴胺的反应.
主要成果:
- ITP+ LNs被确定为早晨突出的神经元.
- 在ITP+ LNs中,Dop1R1的CRISPR/Cas9突变发生显著降低了早晨的活动.
- 多巴胺增加ITP + LNs中的cAMP水平,这是一种依赖Dop1R1的反应,并在早上增强.
- 晨间Dop1R1mRNA水平依赖光线上升调节.
结论:
- 在ITP+ LNs中通过Dop1R1传递多巴胺信号,促进了的早晨活动.
- 光能提高这些神经元中的Dop1R1表达的调节,增强它们对多巴胺的反应.
- 这种机制解释了光如何刺激的早晨清醒,反映了人类的反应.
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