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Updated: Jun 30, 2025

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密集,连续的膜标记和扩展显微镜 组织中超结构的可视化.

Tay Won Shin1,2,3, Hao Wang1,4, Chi Zhang1

  • 1McGovern Institute, Massachusetts Institute of Technology, Cambridge, MA 02139.

bioRxiv : the preprint server for biology
|March 18, 2024
PubMed
概括

超结构膜扩展显微镜 (umEXM) 能够在完整的组织中对脂质膜进行高密度光标记. 这种技术可以实现膜和蛋白质的纳米可视化,这对于理解细胞结构至关重要.

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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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