黑色素调节昼夜时钟蛋白的表达在过敏的气道炎症
Si-Nuo Guo1,2, Xu-Qin Jiang2,3, Ning Chen1,2
1Anhui Geriatric Institute, Department of Geriatric Respiratory and Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China.
Heliyon
|March 18, 2024
概括
黑色素通过调节Per1昼夜时钟蛋白来缓解过敏的呼吸道炎症. 这项研究表明,黑激素.
科学领域:
- 免疫学 免疫学 免疫学
- 时间生物学 时间生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 喘表现出明显的昼夜节律,通常与一个被破坏的分子钟.
- 众所周知,黑素调节昼夜节律,并显示出减轻喘症状的潜力.
研究的目的:
- 在喘模型中研究黑激素对昼夜钟蛋白质表达的影响.
- 为了确定黑激素的抗喘作用是否与其对生理时钟的调节有关.
主要方法:
- 在使用卵胺 (OVA) 挑战的雌性C57BL/6小鼠中确立过敏呼吸道炎症.
- 服用黑激素或黑激素受体抗剂Luzindole,以评估对炎症和时钟蛋白表达的影响.
- 分析了呼吸道炎症,粘液分泌和昼夜蛋白质 (Bmal1,Per1,Clock,Timeless,Cry1,Cry2),黑激素合成酶 (ASMT,AANAT) 和黑激素受体 (Mel-1A/B-R) 的蛋白质水平.
主要成果:
- OVA挑战导致炎症细胞透的增加和肺组织中昼夜钟蛋白的表达升高.
- 过敏小鼠显示ASMT表达和黑激素水平降低,但黑激素受体表达增加.
- 黑色素治疗改善了呼吸道炎症并增加了Per1和Clock的表达;卢辛多尔加剧了炎症并改变了时钟蛋白的表达.
结论:
- 循环时钟蛋白表达在过敏呼吸道炎症期间在肺部增加.
- Per1被确定为一种由外源和内源黑激素调节的关键昼夜蛋白质.
- 在缓解Th2型气道炎症方面,Per1代表了黑激素的潜在治疗标.
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