相关实验视频

Updated: Jun 30, 2025

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

Published on: November 5, 2013

通过氧化酸减少与ABTS氧化相结合,对抗氧化能力的色度检测

Burcu Bekdeşer¹, Reşat Apak^1,2

¹Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpaşa, Avcilar, 34320 Istanbul, Turkey.

|March 18, 2024

概括

一种新的色度测定方法,即 Auric 降低抗氧化能力 (AuRAC),通过监测黄金纳米粒子形成来测量总抗氧化能力. 这种敏感的方法准确地量化了各种样本中的抗氧化剂,提供了增强的检测极限.

科学领域:

分析化学分析化学
生物化学生物化学
材料科学材料科学材料科学

背景情况:

准确测量总抗氧化能力 (TAC) 对健康和食品科学至关重要.
现有的TAC色度测试通常缺乏灵敏度或具有有限的线性范围.
染色体试剂ABTS (2,2'-azinobis(3-乙基索醇-6-硫酸)) 在抗氧化剂测定中被广泛使用.

研究的目的:

为测量总抗氧化能力 (TAC) 开发一种简单,敏感和可靠的色度测试方法.
建立一种名为Auric Reducing Antioxidant Capacity (AuRAC) 的新方法,该方法基于黄金降解.
为了验证AuRAC测定对复杂矩阵的性能和适用性.

主要方法:

开发一种色度测定方法,利用抗氧化剂减少黄金,从而导致黄金纳米粒子 (AuNP) 的形成.
测量氧化ABTS基 (ABTS·+) 的吸收率下降,作为抗氧化活性指标.
使用各种抗氧化剂,合成混合物,茶叶注入剂和药品样本进行AuRAC测定的应用和验证.

主要成果:

AuRAC试验表明,抗氧化剂度与颜色变化/吸收率下降之间存在明显的相关性.
该试验与参考CUPRAC (铜降低抗氧化能力) 试验相容,并表现出良好的线性,精度和恢复.
与传统的单电子转移 (SET) 试验相比,AuRAC在较低的抗氧化剂度和较高的摩尔吸收系数下提供了更平滑的线性范围.

结论:

开发的AuRAC测定是一种可靠和强大的方法,用于定性和定量确定总抗氧化能力.
该测试的高灵敏度,源于由于黄金的3电子减小而增加的摩尔吸收性,使其优于许多现有方法.
AuRAC测定显示了将其转换为纸质色度传感器的巨大潜力,可广泛应用.

更多相关视频

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

Published on: June 10, 2022

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

相关概念视频

Redox Titration: Overview

Redox Titration: Overview

Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...

Redox Titration: Other Oxidizing and Reducing Agents

Redox Titration: Other Oxidizing and Reducing Agents

Besides iodine, other oxidizing or reducing agents can serve as titrants in redox titrations. Common oxidizing titrants include KMnO4, cerium(IV), and K2Cr2O7. The choice of oxidizing titrants depends on factors like stability, cost, analyte strength, and reaction rate between the analyte and titrant. KMnO4 is a strong oxidizing titrant that reduces from Mn(VII) to Mn(II) in a highly acidic solution, simultaneously oxidizing the analyte to a higher oxidation state. In this case, KMnO4 acts as a...

Redox Titration: Iodimetry and Iodometry

Redox Titration: Iodimetry and Iodometry

Iodometry and iodimetry are analytical methods used to determine the concentration of oxidizing or reducing agents using iodine. In iodometric titrations, the oxidizing analyte solution is usually acidified and treated with an excess of iodide ions, which generates an equivalent amount of iodine in equilibrium with triiodide. The released iodine is subsequently titrated directly against a standardized reducing agent. As the dilute iodine color becomes pale yellow, a few drops of freshly...