猪中料效率的不同选择改变了十二指肠对料摄入的转录基因反应及其DNA甲基化概况
Guillaume Devailly1, Katia Fève1, Safia Saci1
1GenPhySE, Université de Toulouse, INRAE, ENVT, Castanet Tolosan, France.
Physiological genomics
|March 18, 2024
概括
在猪中提高料效率的遗传选择改变了十二指肠.
科学领域:
- 动物遗传学动物遗传学
- 营养生理学 营养生理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 猪的料效率会影响生产成本和环境足迹.
- 之前的研究发现了低 (LRFI) 和高 (HRFI) 剩余料摄入量猪系之间的食行为和代谢差异.
- 临近肠在营养感知和吸收中的作用被假设为这些差异的基础.
研究的目的:
- 在禁食和养条件下研究LRFI和HRFI猪的十二指肠转录组和DNA甲基化概况.
- 确定十二指肠中与基因选择相关的分子机制,以提高料效率.
- 评估短期料摄入对十二指甲状腺基因表达和甲基化的影响.
主要方法:
- 不同的遗传选择在10代的时间里创造了LRFI和HRFI猪系.
- 对十二指肠组织进行了转录组和DNA甲基化概况分析.
- 在24头猪中,进行了一夜禁食和任意养状态之间的比较.
- 生物信息分析确定了差异表达的基因和甲基化区域.
主要成果:
- 在LRFI和HRFI系之间发现了1,106个差异表达的基因,影响了跨膜运输和细胞分裂.
- 与HRFI线相比,LRFI线对料摄入呈现出更明显的转录基因反应.
- 短期食改变了参与合成体和代谢的基因,包括rRNA产生和自.
- 营养载体和紧结基因在线和/或养状态之间表达差异.
- 在线之间发现了409个不同甲基化的区域,食的影响最小.
结论:
- 对料效率的遗传选择显著改变了猪的十二指甲体转录组概况.
- 十二指肠对料摄入的反应是一个关键因素,受料效率选择的影响.
- 这些发现表明,十二指肠在猪的料效率背后的生理机制中起着至关重要的作用.
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