相关实验视频

Updated: Jun 30, 2025

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

乳腺癌患者的miR-497-5p表达与临床病理学特征和预后之间的相关性

Zhiying Zhang¹, Ying Zhou², Shujing Liang³

¹Department of Hematology and Blood and Marrow Transplantation.

Applied immunohistochemistry & molecular morphology : AIMM

|March 18, 2024

概括

乳腺癌组织中miR-497-5p的低表达与预后较差和死亡风险增加有关. 这种微RNA作为乳腺癌患者死亡的独立预测器.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

乳腺癌 (BC) 是一种异质性疾病,具有多样化的生物和组织学特征.
微RNAs (miRNAs) 在癌症的发展和进展中起着关键作用,影响患者的预后.
了解特定的miRNA角色,如miR-497-5p,对于改善乳腺癌管理至关重要.

研究的目的:

研究乳腺癌患者的miR-497-5p表达和临床病理学特征之间的关联.
评估miR-497-5p在预测乳腺癌总生存率方面的预后价值.
确定miR-497-5p是否作为乳腺癌死亡率的独立风险因素.

主要方法:

在140个乳腺癌组织和相邻的正常组织中,使用逆转录-定量聚合酶链反应 (RT-qPCR) 量化分析miR-497-5p表达.
在miR-497-5p表达水平和临床病理特征 (年龄,瘤大小,等级,阶段,淋巴结转移) 之间的相关性分析.
生存分析和多变量考克斯回归建模,以评估miR-497-5p对患者预后的影响及其作为死亡的独立风险因素的作用.

主要成果:

与正常的邻近组织相比,乳腺癌组织中的miR-497-5p显著下调.
较低的miR-497-5p表达与较大的瘤大小,晚期临床阶段和淋巴结转移相关.
患有低miR-497-5p表达的患者的整体存活率显著下降.

结论:

乳腺癌组织中miR-497-5p的下调与不良的临床病理特征有关.
低miR-497-5p表达是乳腺癌患者预后不佳和生存率降低的重要预测因素.
miR-497-5p代表了乳腺癌死亡率的独立风险因素,突出了其作为治疗标或生物标记物的潜力.

更多相关视频

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...