相关实验视频

Updated: Jun 30, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

MYB/LINC00092调节轴促进了乳头甲状腺癌的进展

Lian Cheng^1,2, Xian Deng^3,4, Yuan Le⁵

¹Department of General Surgery (Thyroid Surgery), Affiliated Hospital of Southwest Medical University, Southwest Medical University, Luzhou, China.

Endokrynologia Polska

|March 18, 2024

概括

长非编码RNA LINC00092 作为状甲状腺癌 (PTC) 的瘤抑制剂. MYB通过直接或通过miR-4741.1抑制LINC00092来促进PTC进展.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
在瘤学瘤学.

背景情况:

甲状腺癌是一种普遍存在的内分泌恶性瘤.
乳头甲状腺癌 (PTC) 的进展涉及复杂的分子机制.
长非编码RNAs (lncRNAs) 在癌症发展中起着关键作用.

研究的目的:

为了阐明LINC00092在乳头甲状腺癌 (PTC) 进展中的分子机制.
在PTC中调查MYB,LINC00092和miR-4741之间的监管关系.
确定LINC00092在PTC中作为潜在的瘤抑制剂的作用.

主要方法:

在癌症基因组图集中的LINC00092表达的分析甲状腺癌 (TCGA-THCA) 患者队列和PTC细胞系.
功能测试包括MTT和伤口愈合测试,以评估细胞增殖和迁移.
染色体免疫沉 (Q-ChIP) 和光酶记者测定用于验证转录和miRNA-mRNA标.

主要成果:

发现LINC00092表达在甲状腺癌中被抑制,并且与高级TNM阶段负面相关.
LINC00092抑制了皮质-介质细胞过渡 (EMT),迁移和PTC细胞的增殖.
MYB被确定为一种直接抑制LINC00092的转录因子,并且还诱导miR-4741,这反过来抑制LINC00092,形成一个促进EMT的MYB-miR-4741-LINC00092轴.

结论:

在PTC中,LINC00092作为瘤抑制器IncRNA的功能.
MYB-LINC00092轴,涉及MYB (部分通过miR-4741) 对LINC00092的直接和间接压制,有助于PTC的进展.
包括MYB,LINC00092和miR-4741在内的连贯的前循环调节了PTC的发展.

关键词:

现在我们来看一下LINC0009292的情况.这就是MYB.在 miR-474141 里面.皮囊甲状腺癌是甲状腺癌.

更多相关视频

Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma

Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma

Published on: June 2, 2023

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Published on: February 3, 2023

相关概念视频

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

The Retinoblastoma Gene

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...

The Intrinsic Apoptotic Pathway

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

The Nucleolus

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...