在水培条件下测量Arabidopsis thaliana根的细菌殖民
Xia Shu1, Huatai Li2, Jing Wang3
1State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences; State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University.
Journal of visualized experiments : JoVE
|March 18, 2024
概括
一种标准化的方法量化了Arabidopsis thaliana根的细菌殖民. 这种可复制的技术通过准确测量树根球细菌来增强植物微生物相互作用研究.
科学领域:
- 植物与微生物的相互作用
- 微生物学 微生物学
- 植物科学 植物科学
背景情况:
- 植物根的细菌殖民对于植物微生物相互作用至关重要.
- 在量化根球细菌的可复制性对于可靠的实验结果至关重要.
- 现有的测量根植殖的方法缺乏标准化.
研究的目的:
- 开发和介绍一种标准化,可重复的方法,用于量化阿拉比多普西斯·塔利亚纳菌根圈中的细菌殖民.
- 为研究植物微生物相互作用的研究人员建立一个可靠的协议.
主要方法:
- 无菌的Arabidopsis thaliana是通过水培养培养的,并用细菌接种 (OD600的0.01).
- 在注射后2天收获根,冲洗,称重,并通过旋转提取殖民的细菌.
- 细菌悬浮液在酸盐缓冲盐水 (PBS) 中连续稀释,涂上卢里亚-贝尔塔尼 (LB) ,在37°C化10小时,并计数殖民地.
主要成果:
- 描述的方法允许量化殖民Arabidopsis thaliana根的细菌细胞.
- 殖民地数量被规范化,以表示根系球中的细菌殖民水平.
- 该方法在单相互作用条件下显示出良好的可重现性.
结论:
- 已经建立了一种标准化和可复制的方法来测量Arabidopsis thaliana根的细菌殖民.
- 该协议有助于准确量化树枝球细菌,提高了植物微生物相互作用研究的可靠性.
- 该方法适用于检测受控,单次相互作用的实验设置中的细菌殖民.
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