通过使用Deinococcus radiodurans的Pprl进行工程改造的复合性Pseudomonas putida增强了混乱类耐受性和 (S) -2-基烯生产
Reihaneh Kordesedehi1, Azar Shahpiri2, Mohammad Ali Asadollahi1
1Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Microbial biotechnology
|March 18, 2024
概括
从Deinococcus radiodurans引入一个调节剂,增强了Pseudomonas putida的作用.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 合成生物学 合成生物学
背景情况:
- Pseudomonas putida ATCC 12633 生物转化化物成有价值的化合物,如 (S) -2-基烯.
- 该菌株对杂热性化物耐受性较差,限制了有效的生物转化.
- 提高应力耐受性对于工业应用至关重要.
研究的目的:
- 为了提高P. putidaATCC 12633.的耐压力和生物转化效率.
- 研究Deinococcus radiodurans的全球调节剂PprI在赋予交叉保护中的作用.
- 使用工程P. putida. 优化2-基烯的生产.
主要方法:
- 来自D. radiodurans的编码PprI的基因在P. putidaATCC 12633中使用LacI^Q/P_trc表达系统来表达.
- 评估了工程菌株对各种应力 (化物,乙醇,NaCl,H2O2,热) 的耐受性.
- 工程菌株的整体休息细胞被用于用甲和甲生产2-基烯.
主要成果:
- 改造的P. putida菌株对多种压力表现出显著增强的耐受性,包括甲,乙甲,乙醇,丁醇,NaCl,H2O2和热压力.
- 与父菌株相比,该菌株在将化物转化为2-基烯的效率有所提高.
- 在优化条件下,在3小时内达到1.6g/L的2-基烯的度,产品产量和生产率提高了35%.
结论:
- 从D. radiodurans中引入PprI有效地提高了P. putidaATCC 12633.ATCC 12633.中的混乱耐受性.
- 经过工程改造的表达PprI的P. putida菌株表现出改善的生物转化能力,用于生产2-基烯.
- 这项研究为开发强大的微生物细胞工厂用于工业生物转化提供了一个有希望的战略.
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