多功能光开源体内/体外成像系统 (openIVIS)
John M Branning1,2, Kealy A Faughnan3, Austin A Tomson4
1Quantitative Biosciences and Engineering, Colorado School of Mines, Golden, Colorado, United States of America.
PloS one
|March 18, 2024
概括
研究人员使用Raspberry Pi和3D打印开发了一种低成本的开源光成像系统 (openIVIS). 这种多功能仪器可以进行从实验室测试到现场应用的各种科学测量,展示了可访问技术在研究和教育中的力量.
科学领域:
- 生物光子学 生物光子学
- 开源硬件和软件是开源的.
- 低成本的科学仪器仪表.
背景情况:
- 开源微控制器和3D打印使科学工具更容易获得.
- 现有的低成本仪器包括显微镜,光谱仪和传感器.
- 这些设备支持跨科学学科的研究,教育和实验.
研究的目的:
- 设计和建造一个多功能光开源体内/体外成像系统 (openIVIS).
- 为了整合树派,商业相机,LED和3D打印组件.
- 为了使可见光谱中的光测量能够使用三个激发波长.
主要方法:
- 集成了一个树派与商业摄像机和LED.
- 使用3D打印结构和烯酸外.
- 开发了用于光测量的Python控制软件.
主要成果:
- 开放IVIS系统在各种实验中展示了性能,包括实验室测试和可现场应用.
- 成功拍摄植物,矿物质的图像,并在十天内跟踪小动物的发育.
- 在可见波长中测量了光反应的广泛动态范围 (millimolar到picomolar).
结论:
- 开源硬件和软件,结合可定制制造,可以创建多功能,低成本的科学仪器.
- 开放IVIS系统为研究和教育环境提供了一个灵活而强大的工具.
- 这项研究提出了一个模型,用于开发可访问的科学仪器,用于更广泛的科学探索.
相关概念视频
01:16Confocal Fluorescence Microscopy
13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K
01:37Super-resolution Fluorescence Microscopy
7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K