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通过KIF5B介导的FMDV内部化促进了病毒感染

Wei Zhang¹, Fan Yang¹, Yang Yang¹

¹State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China; Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou 730046, China.

Virologica Sinica

|March 18, 2024

概括

素家族成员5B (KIF5B) 对于口疫病毒 (FMDV) 进入细胞至关重要. 这一发现为开发针对FMDV的抗病毒药物提供了潜在的新目标.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

口病 (FMD) 是一种具有重大经济影响的高度传染性病毒性疾病.
尽管已确定其细胞受体,但口病病毒 (FMDV) 进入细胞的确切机制仍然不完全理解.

研究的目的:

阐明FMDV内部化到宿主细胞的基础分子机制.
为了确定参与FMDV感染和复制的宿主因素.

主要方法:

同免疫沉 (Co-IP) 证实蛋白相互作用.
在受感染细胞中的同局部化研究.
KIF5B 过度表达,淘汰和淘汰的实验.
对FMDV复制和病毒颗粒传输的分析.

主要成果:

基尼辛家族成员5B (KIF5B) 被确定为FMDV内部化的关键宿主因素.
证实了KIF5B和FMDV结构蛋白VP1之间的直接相互作用,特别是涉及KIF5B茎域.
KIF5B过度表达增强了FMDV复制,而KIF5B枯竭抑制了它.
KIF5B调节了克拉的脱涂,并促进了病毒颗粒的传输到内分泌体,促进了FMDV的进入.

结论:

KIF5B在FMDV内部化中发挥着关键作用,通过调节克拉脱涂和细胞内运输来调节FMDV内部化.
KIF5B和FMDV VP1之间的相互作用对于有效的病毒进入和复制至关重要.
KIF5B代表了一种潜在的新型治疗点,用于疾病毒抗病毒药物开发.

关键词:

克拉特林 (Clathrin) 是一个名为克拉特林的词汇.内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌在FMDV中使用FMDV.KIF5B KIF5B KIF5B KIF5B KIF5B KIF5B KIF5B KIF5B KIF5B KIF5B KIF5B KIF5B 这是一种VP1蛋白质.

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