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一种基于悬浮微阵列上的同热放大高通量DNA分析方法,用于检测mopox病毒和具有相似症状的病毒

Liming Zhang¹, Jieyu Liu¹, Shisi Huang²

¹Key Laboratory of Tropical Molecular Pharmacology and Advanced Micro/Nano Diagnostic Technology, School of Tropical Medicine, Institute of Micro and Nanotechnology, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, 571199, China.

Analytica chimica acta

|March 18, 2024

概括

一种新的高通量测定方法结合了悬浮微阵列和同热放大,用于快速检测mpox病毒 (MPXV) 和类似病毒. 这种敏感且具有成本效益的方法有助于区分MPXV与其他具有重叠症状的病毒感染.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断
病毒学病毒学

背景情况:

蒙波克斯病毒 (MPXV) 感染的症状与其他病毒感染相似,如B病毒 (BV),简单疹病毒 (HSV-1,HSV-2) 和疹病毒 (VZV).
仅仅基于临床症状来区分MPXV和这些病毒是具有挑战性的,因为症状重叠.

研究的目的:

开发一种高通量,敏感和定量方法来检测MPXV和其他具有类似临床表现的病毒.
克服MPXV和其他病毒病原体之间的症状重叠所带来的诊断局限性.

主要方法:

组合悬浮微阵列技术与复合酶辅助放大 (RAA) 进行核酸检测.
在珠子阵列上利用同热放大用于信号放大和检测.

主要成果:

实现高灵敏度,检测极限 (LOD) 为0.1 fM,线性范围为0.3 fM至20 pM.
证明了精致的选择性,检测仅取决于目标病毒核酸的存在.
获得可靠的血清恢复 (80.52%119.09%) 和DNA提取后1小时的快速检测时间.

结论:

开发的试验具有成本效益,时间效率,高度灵敏,并提供高通量能力.
这种创新方法可用于检测MPXV和其他具有类似症状的病毒.
该测试作为临床诊断,食品安全和有害微生物环境监测的有效工具.

关键词:

细胞计数珠数组是指细胞计数珠数组.检测 DNA 检测 DNA 的检测.高通量分析的分析.异热放大器的同热放大方式蒙博克斯病毒 (Mopox virus) 是一种病毒.

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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...