相关实验视频

Updated: Jun 30, 2025

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis

Published on: August 21, 2020

FOXO1调节了人类牙纤维细胞的伤口愈合反应

Leticia Rojas^1,2, Nicolás Tobar³, Javier Espinoza¹

¹School of Dentistry, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.

Journal of periodontal research

|March 19, 2024

概括

叉头盒-O 1 (FOXO1) 在伤口愈合中对牙纤维细胞功能至关重要. 抑制FOXO1会减少肌纤维细胞的分化,迁移和原的收缩,这些过程对组织修复至关重要.

科学领域:

口腔生物学口腔生物学
伤口愈合研究研究研究.
纤维细胞生物学

背景情况:

众所周知,叉头盒-O 1 (FOXO1) 在口腔伤口愈合过程中参与上皮质屏障功能.
它在连接组织区,特别是纤维细胞活动中的作用不太清楚.

研究的目的:

研究FOXO1在调节纤维细胞活性中的作用,这与伤口愈合有关.
探索FOXO1在肌纤维细胞分化和细胞外矩阵重塑中的参与.

主要方法:

人类牙纤维细胞被培养并用FOXO1抑制剂治疗.
试验包括肌纤维细胞分化,原凝收缩,细胞迁移,扩散和整合素激活.
基因和蛋白质表达 (α-SMA,纤维素,原I,TGF-β1,β1整合素) 通过qRT-PCR和西欧涂抹分析.
免疫光和免疫组织化学被用来评估蛋白质分布和表达在体外和体外小鼠模型.

主要成果:

抑制FOXO1降低了α-SMA表达,并降低了纤维素,原I,TGF-β1和β1整合素的mRNA水平.
抑制FOXO1导致细胞迁移,扩散,原体凝收缩和β1整合素激活的减少.
在小鼠牙伤床内的纤维细胞中,FOXO1和TGF-β1的表达高.

结论:

在牙纤维细胞中,FOXO1显著调节关键的伤口愈合功能.
在牙肌纤维细胞分化,细胞迁移和原体收缩中,FOXO1起着关键的调节作用.
这些发现突显了FOXO1在组织修复和纤维化过程中的重要性.

关键词:

原蛋白是一种原蛋白.结合组织结合组织.整合物是一种整合物.转录因子是一种转录因子.

更多相关视频

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

Published on: March 25, 2020

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Overview of Regeneration and Repair

Overview of Regeneration and Repair

Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...