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通过基于离子添加的超高性能液体染色学-协同质谱法辅助尿路类固醇的分析

Yue Pan¹, Qiuyi Wang¹, Mengyao Chen¹

¹Institute for Protein Research, Osaka University, Osaka, Japan.

Rapid communications in mass spectrometry : RCM

|March 19, 2024

概括

基于离子添加的超高性能液体色谱-并联质谱 (UHPLC/MS/MS) 增强了尿液分析中的3类固醇检测灵敏度. 与传统技术相比,这种方法可以更好地检测出13种特定的3-OH类固醇.

科学领域:

分析化学分析化学
生物化学生物化学
质谱测量质量谱测量

背景情况:

传统的电子喷雾电离方法由于脱水而降低了对3-基类固醇的检测灵敏度.
基于离子添加的UHPLC/MS/MS是为了克服脱水问题和改善3-OH类固醇检测而开发的.

研究的目的:

使用优化的UHPLC/MS/MS方法,对人类尿液中的总和自由类固醇进行分析.
为了比较离子添加 (Li方法) 与常规质子离子检测 (H方法) 对3-OH类固醇的灵敏度.

主要方法:

尿样被处理为使用固相提取 (SPE) 进行自由和完全的类固醇隔离.
超高性能液态染色学-并联质谱学 (UHPLC/MS/MS) 用于+加和常规质子离子检测.
使用特定的标准曲线进行了3-OH类固醇和其他类固醇的量化.

主要成果:

与传统的H方法相比,Li+添加方法在检测3-OH类固醇方面表现出显著更高的灵敏度.
对男性和女性尿液的分析揭示了与性类固醇相关的明显差异.
类固醇水平正常化为肌素,以便进行准确的比较.

结论:

在有限的尿量中确定了29个总和19个自由类固醇.
基于Li+添加的UHPLC/MS/MS方法有效地改善了13种特定的3-OH类固醇的检测.
这种先进的质谱技术为生物样本中的类固醇分析提供了更高的灵敏度.

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