使用从Saccharolobus solfataricus STB09中获得的葡萄糖分枝酶,增强β-环氧德克斯的生产
Luxiao Wang1,2, Shuangdi Chen1,2, Caiming Li3,1,2
1School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Journal of agricultural and food chemistry
|March 19, 2024
概括
这项研究引入了一种新的方法,通过使用糖原脱枝酶 (SsGDE) 来分解粉分支来促进β-环氧素 (β-CD) 的产生. 这种方法显著提高了β-CD产量,提供了更有效的酶性生产途径.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 碳水化合物化学 碳水化合物化学
背景情况:
- 粉的α-1,6葡萄糖酸键阻碍了有效的循环氨酸 (CD) 生产,它们会干扰循环氨酸糖基转移酶 (CGTase) 活性.
- 目前用于CD合成的方法面临的挑战是由于基质的限制,最大限度地提高产量.
研究的目的:
- 研究使用来自*Saccharolobus solfataricus*STB09的葡萄糖脱枝酶 (SsGDE) 来增强β-环氧素 (β-CD) 的产生.
- 探索SsGDE在酶合成中改善β-CD产量的机制.
主要方法:
- 在粉加工的液化阶段加入SsGDE.
- 使用SsGDE和β-循环二烯糖酶转移酶 (β-CGTase) 的组合对粉进行酶性处理.
- 分析分支后的副产品产生和链条长度分布.
主要成果:
- 添加SsGDE (750U/g粉) 显著增加了β-CD产量43.9%.
- SsGDE选择性地化了α-1,6葡萄糖酸键,最大限度地减少了副产品的形成.
- 聚合度≥13的脱枝粉链被β-CGTase有效地转化为CD.
结论:
- 将SsGDE与β-CGTase结合在一起,为显著增强酶性β-CD产生提供了一个有希望的策略.
- 通过优化粉基质对CGTase的可访问性,ssGDE促进了改善β-CD产量.
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