在地下水中高多样性,丰富性和化酶的表达
Shengjie Li1,2, Damon Mosier1, Angela Kouris1
1Department of Earth, Energy and Environment, University of Calgary, Calgary, AB T2N 1N4, Canada.
ISME communications
|March 19, 2024
概括
地下地下水中存在各种各样的酶酶,这些酶对能量代谢至关重要. 这些[NiFe]-基酶丰富且活跃表达,表明在这些环境中氧化具有显著的潜力.
科学领域:
- 微生物生态学 微生物生态学
- 地质化学 地质化学
- 分子生物学分子生物学
背景情况:
- 是地下环境中一个关键的电子捐赠体.
- 了解化酶的作用对于地下微生物生态至关重要.
- 之前的研究对地下水中酶多样性和活性的数据有限.
研究的目的:
- 分析各种地下水中的化酶的多样性,丰富性和表达.
- 为了扩大[NiFe]-基酶的已知序列.
- 为了研究不同酶组的生态作用.
主要方法:
- 分析了5个蛋白质组,25个元基因组和265个地下水片数据集.
- [NiFe]-酶基因的恢复和测序.
- 生物信息分析酶基因的丰富性,表达和家族遗传组.
主要成果:
- 1545个新的[NiFe]-酶基因序列被恢复,大大增加了数据库条目.
- 基酶非常丰富 (可与RNA聚合酶相比较),并且活跃表达,包括多达5.9%的甲原蛋白质组.
- 酶基因的丰富性随着深度的增加而增加,3d组是最丰富的,在甲类和化疗类中发现;3a组在蛋白质组中最丰富;3b组与硫代谢有关;确定了两个新组.
结论:
- 地下水中存在广泛而多样化的酶群体.
- 化酶在地表微生物代谢和能量循环中发挥着重要作用.
- 这些发现表明,在地下息地,氧化能力很高.
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