使用三重元编码方法在沉积物中区分活跃,休眠和死亡的微核细胞
Huiwen Deng1,2, Cui He1, Alexandra Z Worden3,4
1School of Marine Sciences, Sun Yat-sen University, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China.
Environmental microbiology
|March 19, 2024
概括
区分活跃,休眠和死亡的微生物对于理解微生物群落至关重要. 这项研究开发了一种方法来区分这些状态,揭示了沉积物中显著的休眠和遗留的微核细胞群.
科学领域:
- 环境微生物学环境微生物学
- 分子生态学分子生态学
- 超标编码技术 (Metabarcoding) 是一种代码编码技术.
背景情况:
- 环境DNA (eDNA) 代码化通常包括来自生物和死生物的DNA.
- 区分活跃,休眠和死亡的微生物细胞对于准确的社区特征是必不可少的.
- 以前的方法专注于活跃细胞和死亡细胞,但将休眠细胞与活跃细胞区分开来仍然是一个挑战.
研究的目的:
- 开发和应用一种新的方法,将微生物群落分为活跃,休眠和遗留部分.
- 在不同的湿度条件下,研究潮间沉积物中的微核细胞分数的多样性和组合结构.
- 评估环境驱动因素对微生物活动状态动态的影响.
主要方法:
- 对rRNA和rDNA进行测序,分别评估活性和总微生物池.
- 使用 propidium monoazide (PMA) 交叉链接的 rDNA 来专门准可活细胞中的 DNA.
- 将这些方法结合起来,在环境样本中区分和量化活跃,休眠和遗留的微核细胞分子.
主要成果:
- 在总rDNA中,微核细胞类型的很大一部分来自休眠 (13%的丰富度,20%的丰富度) 和遗留 (47%的丰富度,14%的丰富度) 细胞.
- 休眠微核细胞的丰富性和丰富性在从潮湿到干燥条件的过渡期间显著增加.
- 环境驱动因素被确定为影响不同微生物活性分数的多样性和组合结构的关键因素.
结论:
- 开发的策略有效地区分了环境样本中的活跃,休眠和遗留微核细胞分子.
- 沉积物微生物群落有大量的休眠和遗留物种群,影响了整体的多样性评估.
- 这种多功能方法可以适应在各种环境中对各种微生物群落进行表征.
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