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转录因子BpTCP20通过乙转移酶BpPDCE23的乙化调节了树的盐耐受性
Zhujun Liu1, Xinxin Shi1, Zhibo Wang1
1State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Plant physiology
|March 19, 2024
概括
伯奇BpTCP20通过调节口腔关闭和减少活性氧物种来提高盐分耐受性. 通过BpPDCE23的乙化促进了BpTCP20的增强.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 泰奥辛特分支1/环状体/增殖细胞因子 (TCP) 转录因子参与非生物应激反应.
- TCPs对盐分耐受性的贡献的具体机制在很大程度上是未知的.
研究的目的:
- 为了研究BpTCP20的作用,从树 (Betula platyphylla Suk) 的TCP转录因子,在赋予盐耐受性.
- 阐明BpTCP20介导的盐耐受性背后的分子机制.
主要方法:
- 在树中通过过度表达和淘汰突变分析对BpTCP20的表征.
- 通过监测生理参数,如口腔关闭和反应性氧物种 (ROS) 水平来评估盐分耐受性.
- 染色体免疫沉测序 (ChIP-seq) 用于识别BpTCP20结合标.
- 位点定向突变发生,以研究 lysine 259 的乙化作用.
- 同免疫沉测试以确定相互作用的蛋白质,包括乙转移酶.
主要成果:
- 过度表达BpTCP20增强了树的盐耐受性,而Bptcp20淘汰突变体的耐受性降低.
- BpTCP20通过调解口腔关闭和减少ROS积累而起作用.
- ChIP-seq确定了NeuroD1,T-box,TBS1和TBS2作为BpTCP20的直接结合元素.
- 乙化BpTCP20在lysine 259由BpPDCE23增强其DNA结合和转录活性,对于盐耐受性至关重要.
- BpPDCE23是一种乙转移酶,与BpTCP20相互作用和乙化,积极调节其功能.
结论:
- BpTCP20是白树盐耐受性的一个关键的转录调节器.
- 翻译后的修改,特别是BpPDCE23的乙化,在调节BpTCP20活性和盐分耐受性方面发挥着关键作用.
- 了解BpTCP20法规提供了关于植物适应盐水环境的见解.
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