人类呼吸道病毒RNA在废水沉积固体中的持久性
Mengyang Zhang1, Laura Roldan-Hernandez1, Alexandria Boehm1
1Department of Civil and Environmental Engineering, School of Engineering and Doerr School of Sustainability, Stanford University, Stanford, California, USA.
Applied and environmental microbiology
|March 19, 2024
概括
对呼吸道病毒的废水监测显示,废水固体中的病毒RNA衰变有限,比典型的运输时间减少0-20%. 这些发现有助于基于废水的流行病学数据的解释.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生监督是对公共卫生的监督.
- 分子病毒学分子病毒学.
背景情况:
- 基于废水的流行病学 (WBE) 使用核酸度监测社区病毒性疾病.
- 废水中呼吸道病毒核酸的命运,包括降解和吸收,还不清楚.
- 了解废水中的病毒RNA稳定性对于准确的WBE数据解释至关重要.
研究的目的:
- 在废水固体中研究来自7种人类呼吸道病毒和胡轻斑病毒 (PMMoV) 的基因组核酸的衰变动力学.
- 为了确定温度对病毒RNA衰变速率的影响.
- 在典型的废水运输时间内评估病毒RNA衰变的程度.
主要方法:
- 七种呼吸道病毒 (SARS-CoV-2,RSV,HCoV-OC43,HCoV-229E,HCoV-NL63,HRV,IAV) 和PMMoV被入废水固体中.
- 病毒RNA度在50天内在三个温度下 (4°C,22°C,37°C) 进行监测.
- 使用第一阶动力学分析了衰变动力学,确定了衰变速率常数 (k).
主要成果:
- 病毒基因组RNA的衰变遵循一阶动力学,衰变速率常数 (k) 从0到0.219每天.
- 衰变速度取决于温度,在37°C观察到的最大值.
- 在典型的污水停留时间 (<1天) 中,无论温度如何,观察到有限的病毒RNA衰变 (0-20%的减少).
结论:
- 在呼吸道疾病 (WBE) 中使用的病毒RNA标在运输过程中在废水固体中表现出有限的衰变.
- 报告的衰变速率常数可以直接纳入WBE的命运和运输模型.
- 这项研究为改善基于废水的流行病监测的准确性和解释提供了关键数据.
更多相关视频
09:26Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
Published on: June 30, 2023
1.1K
10:53Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
Published on: March 17, 2023
1.5K
相关概念视频
Viruses of Archaea
Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
Viruses with RNA Genomes
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Human Virome
The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...
Reservoir of Infection
Infectious diseases arise from intricate interactions between pathogens and their reservoirs. A reservoir of infection refers to the natural habitat where a pathogen lives, grows, and multiplies, serving as a continual source of infection. Reservoirs are broadly classified as either living or nonliving, and each plays a unique role in disease transmission, significantly influencing public health interventions and control strategies.Humans act as reservoirs for a wide array of pathogens,...
Poliomyelitis
Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...
Respiratory Syncytial Virus Disease
Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...
