在COVID-19患者中,类似ACE-2的酶活性与免疫球蛋白有关
Yufeng Song1, Regan Myers1, Frances Mehl1
1Department of Pediatrics, University of Virginia, Charlottesville, Virginia, USA.
mBio
|March 19, 2024
概括
COVID-19患者可能会产生独特的抗体,称为abzymes,模仿酶血管素转化酶2 (ACE2). 这些抗RBD酶可能解释了COVID-19病原体的一些不清楚的方面.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 生物化学 生物化学
背景情况:
- COVID-19呈现出复杂的症状,如自主调节失调和不清楚来源的凝血病.
- SARS-CoV-2 尖端蛋白的受体结合域 (RBD) 针对宿主细胞上的 ACE2 酶.
- 具有酶活性的抗体 (酶体) 与各种疾病有关.
研究的目的:
- 为了调查一些COVID-19患者发展ACE2类非酶的假设.
- 探索这些亚基酶在COVID-19病变发生过程中的潜在作用.
- 描述首例与COVID-19中的免疫球蛋白相关的ACE2类酶活性.
主要方法:
- 分析了住院COVID-19患者的血样本.
- 对合成ACE2基质的裂变进行了血评估.
- 使用大小排除色谱和蛋白质A/G吸附来耗尽免疫球蛋白.
主要成果:
- 一些COVID-19患者的血在合成ACE2基质上表现出类似ACE2的催化活性.
- 这种活性依赖于Zn2+并被EDTA抑制,与金属蛋白酶活性一致.
- 免疫球蛋白耗尽 (>99.99%) 取消了观察到的基质裂变活性.
- 这些发现表明存在具有类似abzyme功能的抗RBD抗体.
结论:
- COVID-19 患者可能会产生具有 ACE2 类亚酶活性的抗体.
- 这些酶可能会导致无法解释的COVID-19症状的发病.
- 需要进一步的研究,以了解抗RBD酶在疾病中的更广泛影响.
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