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Updated: Jun 30, 2025

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

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Published on: January 8, 2012

胎盘 trofhoblast 细胞隔离从术语胎盘的胎盘

Manuel Vidal Jr S^1,2, Radnaa Enkhtuya², Rheanna Urrabaz-Garza²

¹College of Medicine, University of the Philippines Manila, Manila, Philippines.

Methods in molecular biology (Clifton, N.J.)

|March 19, 2024

概括

这项研究提出了一项新的三相协议,用于分离纯粹的,可行的初级人类胎盘囊细胞. 这种方法克服了研究胎盘生物学现有的细胞系的局限性.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
人类胎盘 trofhoblast 研究研究.

背景情况:

现有的胎盘生物学研究的细胞系,如胆 ?? 瘤或不朽的热原体细胞,可能不能准确地代表人类胎盘热原体细胞.
来自长期胎盘的初级状热囊细胞是研究的宝贵来源,但它们的隔离和在延长培养中维持存在挑战.

研究的目的:

开发和描述一个强大的三相协议,用于隔离和扩展培养纯初级人类胎盘热囊细胞.
提供一种可靠的方法来获得与当前细胞系模型相比,更能与体内条件相比较的热囊细胞.

主要方法:

一个三相协议,涉及酶/机械消化,修改的Percoll梯度密度分离和使用磁珠进行免疫净化.
使用长期胎盘作为主要状热囊细胞的来源.

主要成果:

开发的协议产生了高度纯净的热囊细胞培养.
隔离的热细胞在最初通过后长时间保持活力.

结论:

描述的三阶段协议提供了一种有效的方法,用于隔离和保持培养中纯原始人类胎盘 trofhoblasts.
这种改进的方法通过提供更好的细胞模型,促进了对人类胎盘生物学进行更准确的研究.

关键词:

细胞培养培养的细胞培养.胎儿与母亲的接口怀孕怀孕怀孕怀孕术语胎盘胎盘的时间热细胞热细胞

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Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.