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对于单细胞技术而言,胎盘组织的解离

Valeria Garcia-Flores¹, Derek Miller¹, Jose Galaz²

¹Center for Reproductive Health Sciences, Department of Obstetrics and Gynecology, Washington University School of Medicine, St Louis, MO, USA.

Methods in molecular biology (Clifton, N.J.)

|March 19, 2024

概括

这项研究提出了一种方案,用于从人类胎盘组织中制造单细胞悬浮液. 这些高质量的细胞悬浮物适用于先进的单细胞分析,包括单细胞RNA测序.

科学领域:

细胞生物学细胞生物学
基因组学就是基因组学.
生殖医学生殖医学

背景情况:

单细胞悬浮制剂对于单细胞分析至关重要.
人的胎盘组织 (基底板,小,膜) 需要特定的解离方法.
现有的协议可能无法为下游应用提供最佳的细胞生存能力.

研究的目的:

建立一个强大的协议,从各种人类胎盘组织中产生高质量的单细胞悬浮液.
为这些细胞悬浮物的冷保存和回收提供指导方针.
确保已制备的细胞适用于敏感的单细胞欧米技术.

主要方法:

使用优化的酶和机械方法进行组织解离.
针对基底板,胎盘小和胆膜量身定制的特殊协议.
使用冷保护剂和控制速率冷的冷保存技术.

主要成果:

从所有测试的人类胎盘组织类型中成功生成可行的单细胞悬浮液.
在解离后和解后证明了高细胞活力和完整性.
协议兼容性通过下游单细胞RNA测序证实.

结论:

开发的协议提供了一种可靠的方法,用于从人类胎盘中制备单细胞悬浮液.
这种方法支持先进的单细胞研究,使我们能够更深入地了解胎盘生物学.
该协议促进了未来的研究,需要冷保存的胎盘单细胞.

关键词:

基底板是指基底板的基底板.胆 ?? niotic 膜胆 ?? niotic 膜是一种胎盘上的小.单细胞RNA测序的测序方法

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Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.