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相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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相关实验视频

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Fluorescence Activated Cell Sorting of Plant Protoplasts
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对于植物来说smFISH.

Sahar Hani1,2, Caroline Mercier1,3, Pascale David1

  • 1Aix Marseille Univ, CEA, CNRS, BIAM, UMR7265, Saint-Paul lez Durance, France.

Methods in molecular biology (Clifton, N.J.)
|March 19, 2024
PubMed
概括

我们介绍了一种针对植物优化的单分子光在位杂交 (smFISH) 协议. 这种方法可以可视化和量化RNA分子,揭示基因表达和单分子水平的RNA动态.

关键词:
图像成像是一种成像.植物植物植物植物植物.这是一个RNARNARNARNARNA.单个分子单个分子单个分子转录 翻译 翻译偷偷捕捞的鱼类是什么意思

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科学领域:

  • 分子生物学分子生物学
  • 植物科学 植物科学
  • 遗传学 是一个遗传学.

背景情况:

  • 单分子光在位杂交 (smFISH) 对于在单分子水平上可视化和量化RNA至关重要.
  • 它在植物中的应用是有限的,因为特定的杂交和成像挑战.
  • 了解植物中的基因表达和RNA动态需要先进的分子技术.

研究的目的:

  • 为植物研究开发和介绍一个优化的smFISH实验工作流.
  • 为克服在植物组织中应用smFISH技术的现有挑战.
  • 为了能够对植物中的基因表达和RNA动态进行高分辨率分析.

主要方法:

  • 在植物中准备样本的详细协议.
  • 为植物组织优化探针杂交策略.
  • 针对单个RNA可视化的先进信号检测技术.

主要成果:

  • 成功调整smFISH用于植物样本,克服了以前的限制.
  • 在完整的植物细胞中展示了单个RNA分子的可视化和量化.
  • 建立了一个强大的工作流程,用于分析植物单分子水平的基因表达.

结论:

  • 提出的smFISH协议显著增强了对植物基因表达的研究.
  • 这种技术为整个植物的RNA动态和细胞异质性提供了新的见解.
  • 这种工作流对推动植物分子生物学研究具有很大的前景.