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Updated: Jul 27, 2026

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在Arabidopsis thaliana体细胞中的快速DNA-FISH

Olga Raskina1, Ofir Hakim2

  • 1Institute of Evolution, University of Haifa, Haifa, Israel. olga@evo.haifa.ac.il.

Methods in molecular biology (Clifton, N.J.)
|March 19, 2024
PubMed
概括

本研究介绍了一种简化光在位杂交 (FISH) 协议,用于定位植物核中的DNA. 该方法简化了Arabidopsis thaliana的基因组位点检测,提供更快的结果并保留染色质结构.

关键词:
阿拉比多普西斯塔利亚纳.背面的鱼 - 背面的鱼染色体制备剂 染色体制备剂光在现场混合化中的光.阶段间核的原子核.分子细胞遗传学 分子细胞遗传学植物植物植物的植物.

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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...