相关实验视频

Updated: Jun 30, 2025

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

使用纳米孔测序来分析质细胞转录组第一部分:图书馆准备

Sébastien Skiada¹, Alexandra Launay-Avon^1,2, Arnaud Liehrmann^1,2

¹Institute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, CNRS, INRAE, Université Evry, Gif sur Yvette, France.

Methods in molecular biology (Clifton, N.J.)

|March 19, 2024

概括

这项研究提出了一种新的纳米孔测序协议,以充分描述复杂的叶绿体基因表达. 该方法可以识别和量化全长RNA转录异型,克服以前技术的局限性.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
文字转录学 (Transcriptomics) 是一个学科.

背景情况:

由于RNA拼接,编辑和处理,塑性基因表达是复杂的.
传统的方法难以解决多个转录异型.
RNA测序技术有助于更好地理解,但无法量化全长异构体.

研究的目的:

提供一个全面的协议,从全叶RNA编制cDNA库,用于纳米孔测序.
为了能够识别和量化全长的叶绿体RNA转录异型.
为了促进对叶绿体的转录后修饰的研究.

主要方法:

从全叶RNA制备cDNA库的新方案.
将RNA适配器连接到3'端,用于模板切换反转录.
对牛津纳米孔v14化学的cDNA的调整.

主要成果:

一个完整的协议,生产适合从全部叶子RNA进行纳米孔测序的cDNA库.
该协议克服了识别和量化全长转录异型的限制.
能够同时研究多个在质体中的转录后过程.

结论:

开发的纳米孔测序协议为详细分析质细胞基因表达提供了强大的工具.
它允许解决以前无法量化复杂的转录异型.
这一进展对于研究质体的后转录调节的研究人员来说至关重要.

关键词:

塑是一种塑.纳米孔测序的测序在RNA成熟过程中.处理RNA处理RNA的过程在RNA-Seqq.

更多相关视频

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...