阿斯巴拉金-葡萄糖阿马多里化合物:在干果果中形成,表征和分析
Qing Xiao1, Qingrong Huang1, Chi-Tang Ho1
1Department of Food Science, Rutgers, The State University of New Jersey, 65 Dudley Road, New Brunswick, New Jersey 08901, United States.
Journal of agricultural and food chemistry
|March 19, 2024
概括
亚马多利的阿斯帕拉金与葡萄糖 (Asn-Glc-ARP) 的重组产物被合成和特征化. 确定了Asn-Glc-ARP形成的最佳条件,揭示了降解途径,并量化了它在朱果中的存在.
科学领域:
- 食品化学 食品化学
- 有机化学 有机化学
- 分析化学 分析化学
背景情况:
- 梅拉德反应在食品加工中至关重要,影响味道,颜色和营养价值.
- 阿马多里重组产物 (ARP) 是 Maillard 反应中的关键中间体.
- 了解特定ARP的形成和稳定性,如涉及阿斯巴拉金的ARP,对于食品科学至关重要.
研究的目的:
- 为了合成和描述阿马多里阿斯巴拉金与葡萄糖 (Asn-Glc-ARP) 的重新排列产品.
- 为了确定Asn-Glc-ARP积累的最佳反应条件.
- 研究Asn-Glc-ARP的降解途径和稳定性.
主要方法:
- 梅拉德模型反应被用于合成.
- 液体染色学-质谱学 (LC-MS) 和双重质谱学 (MS/MS) 用于识别和分析.
- 用分离和净化技术来确定结构.
主要成果:
- 对于Asn-Glc-ARP积累的最佳条件是100°C在pH 7下40分钟.
- 阿斯巴拉金 (Asn) 降解发生在性条件下,而在酸性,高温环境中以Asp-Glc-ARP为主体的脱化.
- 作为Asn-Glc-ARP的结构被提出为1-deoxy-1-l-asparagino-d-fructose. 的结构.
- 在干果中量化了Asn-Glc-ARP (8.1 ± 0.5 mg/g) 的显著含量.
结论:
- 这项研究成功合成和表征了Asn-Glc-ARP.
- 反应条件显著影响Asn-Glc-ARP的形成和降解途径.
- 在某些条件下,Asn-Glc-ARP是一种稳定的化合物,在朱果中存在大量.
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