相关实验视频

Updated: Jun 30, 2025

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

优化基于TROSY和抗TROSY的¹⁵N CPMG放松分散实验通过阶段循环

Yingxian Cui¹, Yangzhuoyue Jin¹, Yu Hou¹

¹State Key Laboratory of Natural and Biomimetic Drugs, Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.

Journal of magnetic resonance (San Diego, Calif. : 1997)

|March 19, 2024

概括

使用[0013]阶段循环的新CPMG实验改善了生物分子动力学研究. 这些增强的方法提供了对脉冲缺陷的更强的抵抗力,为大分子提供了更可靠的动力和热力学洞察力.

科学领域:

生物物理学的生物物理.
结构生物学结构生物学
核磁共振 (NMR) 光谱学核磁共振 (NMR) 光谱学

背景情况:

CPMG的放松分散研究提供了关于μs-ms时间尺度上的生物分子动态的关键见解.
¹⁵N标签是这些研究的一个常见且具有成本效益的方法.
传统的CPMG实验可能会因为脉冲不完美而出现缺陷.

研究的目的:

开发和验证新的CPMG实验,纳入[0013]阶段循环以提高稳定性.
改进使用N-TROSY和反-TROSY的方法来研究大型生物分子的动力学.
为了减少放松分散测量的脉冲缺陷的工件.

主要方法:

使用[0013]阶段循环方案实施基于TROSY和anti-TROSY的CPMG实验.
综合的数值模拟来评估性能.
实验验证以与已确定的方法进行比较.

主要成果:

新的[0013]阶段循环CPMG实验显示,相对于传统的恒相方法,对脉冲缺陷的抗性显著提高.
模拟和实验数据证实了在更广泛的频率偏移范围内的改进性能.
衍生的交换参数与已建立的CPMG技术的参数保持一致.

结论:

提出的15个CPMG实验与[0013]相循环提供了一个更可靠的方法来研究生物分子动力学,特别是大分子.
这些方法通过减轻脉冲不完美工件,提供更高的准确性.
这些发现有助于对生物分子功能进行更强大的动力学,热力学和结构分析.

关键词:

在CPMG中,CPMG是CPMG.化学物质交换化学物质交换无形的兴奋状态是看不见的.阶段周期阶段周期阶段周期蛋白质动力学蛋白质动力学一个托盘.

更多相关视频

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

相关概念视频

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...

Double Resonance Techniques: Overview

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.

2D NMR: Overview of Homonuclear Correlation Techniques

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...

NMR Spectrometers: Resolution and Error Correction

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...