全球转录组分析显示,沙门氏菌Typhimurium使用酸盐代谢来对抗胆汁压力
Madhulika Singh1, Deepti Chandra1, Sirisha Jagdish1
1Department of Biochemistry, Indian Institute of Science, Bangalore, India.
FEBS letters
|March 19, 2024
概括
沙门氏菌Typhimurium使用酸盐代谢来适应胆汁. 这种适应涉及冷冲击蛋白E (CspE) 和全球调节器Fnr,提高了在胆汁压力下的生存率.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 病原体适应 病原体适应
背景情况:
- 沙门氏菌Typhimurium是一种肠道病原体,以其胆汁耐受性而闻名.
- 寒冷冲击蛋白E (CspE) 在胆汁压力期间对沙门氏菌的生存至关重要.
- 了解适应机制对于控制沙门氏菌感染至关重要.
研究的目的:
- 为了研究沙门氏菌Typhimurium对胆汁压力的适应性反应.
- 确定冷冲击蛋白E (CspE) 在胆汁耐受性中的作用.
- 阐明酸盐代谢在沙门氏菌适应胆汁中的作用.
主要方法:
- 下一代mRNA测序用于分析暴露于胆汁的野生类型和ΔcspE沙门氏菌Typhimurium菌株的转录变化.
- 基因删除和补充实验,以评估Fnr.的功能.
- 测量细胞内亚酸盐含量和活性氧物种.
主要成果:
- 转录分析揭示了胆汁耐受型沙门氏菌中酸盐代谢基因的上调,包括全球调节器Fnr.
- 一个缺乏Fnr (Δfnr) 的突变体表现出对胆汁压力的敏感性增加.
- 补充Fnr减少了反应性氧物种,并改善了胆汁敏感菌株的存活率.
- 酸盐预处理在胆汁存在时增强了沙门氏菌的生长,与细胞内酸盐的增加相关.
结论:
- 酸盐依赖的新陈代谢是沙门氏菌Typhimurium适应胆汁的关键机制.
- CspE和Fnr在调解沙门氏菌对胆汁压力的反应中起着至关重要的作用.
- 向酸盐代谢途径可能是对抗沙门氏菌感染的新策略.
关键词:
在RNA-seqq.沙门氏菌是一种沙门氏菌.哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈代谢过程中的代谢.酸盐酸盐的使用方法压力就是压力,压力就是压力.更多相关视频
10:18A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
Published on: April 25, 2015
10.4K
08:03A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
Published on: March 28, 2017
10.1K
相关概念视频
Microbial Nutrition
2.0K
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
2.0K
Metabolism of Chemolithotrophs
1.3K
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
1.3K
Inorganic Nitrogen Assimilation
938
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
938
Stringent Response in E. coli
533
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
533
Other Stress Responses in Bacteria
593
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
593
Transduction
3.0K
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
3.0K
