相关实验视频

Updated: Jun 30, 2025

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

患有严重烧伤后缩性痕的患者表达不同的长非编码RNA

Ping Zhou¹, Yan Jiang¹, Ao-Ya Liu¹

¹Department of Burns, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, PR China.

Burns : journal of the International Society for Burn Injuries

|March 19, 2024

概括

长非编码RNAs (lncRNAs) 在烧伤诱导的缩性痕中显示出改变的表达. 这些ncRNAs可以作为治疗点,以抑制异常痕形成.

科学领域:

分子生物学分子生物学
皮肤病学皮肤病学
遗传学遗传学是一个

背景情况:

长非编码RNAs (lncRNAs) 与纤维性疾病有关.
对于lncRNAs在烧伤后缩性痕中的特定作用和机制尚不清楚.

研究的目的:

为了研究 lncRNAs 在烧伤造成的过度缩痕中的差异性表达特征.
探索 lncRNA 参与烧伤痕形成的潜在分子机制.

主要方法:

使用芯片技术 (微阵列) 将烧伤痕患者和健康对照人群 (HCs) 的 lncRNA表达特征进行比较.
使用定量逆转录聚合酶连锁反应 (RT-PCR) 验证的微阵列结果.
利用基因本体学 (GO) 和通路分析来确定差异表达的信使RNA (mRNA) 和它们相关的信号通路的功能.

主要成果:

与HC相比,在高性痕中发现了lncRNA和mRNA表达的显著差异,其中有2738个lncRNA和2166个mRNA的差异表达.
RT-PCR结果证实了微阵列发现的可靠性.
途径分析表明,差异表达的mRNAs主要与免疫系统的细胞因子分泌,痕信号传递和MAPK信号传递途径有关.

结论:

在烧伤引起的过度缩性痕中,lncRNA表达特征发生显著变化.
这些差异表达的lncRNAs代表了减轻烧伤患者异常痕发育的潜在治疗标.

关键词:

烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧不同表达式的差异表达式这是一个LncRNARNA.微阵列的微阵列伤口伤口伤口伤口伤口

更多相关视频

A Mouse Model of Mechanotransduction-driven, Human-like Hypertrophic Scarring

A Mouse Model of Mechanotransduction-driven, Human-like Hypertrophic Scarring

Published on: November 29, 2024

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

Published on: November 6, 2018

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Burn Injuries

Burn Injuries

Burn injuries occur when the skin and underlying tissues are damaged due to exposure to heat, electricity, chemicals, radiation, or friction. They can vary in severity, from minor superficial burns to severe deep burns that can be life-threatening.
The damage results in the death of skin cells, which can lead to a massive loss of fluid. Dehydration, electrolyte imbalance, and renal and circulatory failure follow, which can be fatal. Burn patients are treated with intravenous fluids to offset...